Reelin mRNA Expression During Mouse Brain Development
Reelin mRNA Expression During Mouse Brain Development
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DOI：
10.1111/j.1460-9568.1997.tb01456.x
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发表时间：
1997-05
影响因子：
3.4
通讯作者：
S. Schiffmann;B. Bernier;A. Goffinet
中科院分区：
文献类型：
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作者：
S. Schiffmann;B. Bernier;A. Goffinet
利用原位杂交技术，研究了小鼠大脑发育过程中reelin基因的mRNA表达，该基因最有可能负责reelin性状。从胚胎第13天到成熟。在Cajal - Retzius神经元中发现了最高水平的表达，而在嗅球、小脑外颗粒层以及下丘脑分化区、顶盖和脊髓中也发现了高信号，特别是在发育早期。中低水平表达于中隔区、纹状体区、缰核、部分丘脑核（尤其是外侧膝状核）、视网膜和部分髓质中央区网状结构核。奇怪的是，在reeler突变小鼠的径向胶质细胞、皮质板、浦肯野细胞、下橄榄神经元和许多其他特征异常的区域中没有检测到reelin表达。结合其他初步研究，目前的观察结果表明，reelin的作用是间接的，可能是由细胞外基质介导的。大多数数据可以通过假设reelin是一种细胞排斥分子来解释，它可以阻止迁移的神经元入侵富含reelin的区域，从而促进放射状胶质细胞过程的部署和早期结构模式的形成。
Using in situ hybridization, expression of the mRNA for reelin, the gene most probably responsible for the reeler trait, was studied during mouse brain development. from embryonic day 13 to maturity. The highest level of expression was found in Cajal‐Retzius neurons, while a high signal was also seen in the olfactory bulb, the external granular layer of the cerebellum and, particularly at early developmental stages, in hypothalamic differentiation fields, tectum and spinal cord. A moderate to low level of expression was found in the septa1 area, striatal fields, habenular nuclei, some thalamic nuclei, particularly the lateral geniculate, the retina and some nuclei of the reticular formation in the central field of the medulla. Paradoxically, no reelin expression was detected in radial glial cells, the cortical plate, Purkinje cells, inferior olivary neurons and many other areas that are characteristically abnormal in reeler mutant mice. Together with other preliminary studies, the present observations suggest that the action of reelin is indirect, possibly mediated by the extracellular matrix. Most of the data can be explained by supposing that reelin is a cell‐repulsive molecule which prevents migrating neurons from invading reelin‐rich areas, and thus facilitates the deployment of radial glial cell processes and the formation of early architectonic patterns.