Phytochrome A signal transduction 1 and CONSTANS-LIKE 13 coordinately orchestrate shoot branching and flowering in leafy Brassica juncea
Phytochrome A signal transduction 1 and CONSTANS-LIKE 13 coordinately orchestrate shoot branching and flowering in leafy Brassica juncea
复制标题
光敏色素 A 信号转导 1 和 CONSTANS-LIKE 13 协调协调绿叶芥菜的芽分枝和开花
DOI：
10.1111/pbi.13057
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发表时间：
2019-07-01
影响因子：
13.8
通讯作者：
Zhang, Mingfang
中科院分区：
文献类型：
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作者：
Muntha, Sidra Tul;Zhang, Lili;Zhang, Mingfang
分枝是作物产量的一个主要决定因素，并使旺盛的芽生长和生产一个密集的冠层。光敏色素A信号转导1 （Phytochrome A signal transduction 1, PAT1）在光响应中正调控光敏色素A信号转导，但其对分支的影响尚不清楚。在这项研究中，我们定位了PAT1，并揭示了一个以前未知的与叶型芥菜（Brassica juncea）分支和开花相关的作用。当PAT1表达下调时，枝支发生的时间提前，枝支增加，说明PAT1负向调控枝支。此外，下调的PAT1表达逆转了远红光诱导的分支抑制，表明PAT1参与了避光反应。PAT1仅在芽形成后负调控分枝。观察到的PAT1和BRC1之间的相互作用表明，PAT1以依赖于BRC1的方式影响芽的生长。生化和遗传证据表明，PAT1直接与负调控开花的CONSTANS-LIKE 13 （COL13）相互作用，产生的PAT1-COL13复合物介导茎枝分枝和开花。我们的研究结果揭示了光敏色素信号在调控茎枝分枝和开花过程中与开花途径之间的一种新的串音模式。本文提供的数据可能对涉及GRAS家族转录因子PAT1基因编辑的未来研究有用，以提高作物生产力并实现早期收获。
Branching is a major determinant of crop yield, and enables vigorous shoot growth and the production of a dense canopy. Phytochrome A signal transduction 1 (PAT1) positively regulates phytochrome A signal transduction in response to light, but its effects on branching remain unknown. In this study, we mapped PAT1, and revealed a previously unknown role related to branching and flowering in leafy Brassica juncea. Earlier and increased branching was observed when PAT1 expression was down-regulated, implying that PAT1 negatively regulates shoot branching. Additionally, down-regulated PAT1 expression reversed the inhibited branching induced by far-red light, suggesting PAT1 is involved in the shade avoidance response. PAT1 negatively regulated branching only after bud initiation. The observed interaction between PAT1 and BRC1 implied that PAT1 influences bud outgrowth in a BRC1-dependent manner. Biochemical and genetic evidence indicate that PAT1 directly interacts with CONSTANS-LIKE 13 (COL13), which negatively regulates flowering, with the resulting PAT1-COL13 complex mediating shoot branching and flowering. Our findings reveal a new crosstalk modality between phytochrome signalling and flowering pathways during the regulation of shoot branching and flowering. The data presented herein may be useful for future studies involving the editing of the GRAS family transcription factor PAT1 gene to enhance crop productivity and enable earlier harvesting.