CHEMICAL CONTRAST IN X-RAY MICROSCOPY AND SPATIALLY RESOLVED XANES SPECTROSCOPY OF ORGANIC SPECIMENS
CHEMICAL CONTRAST IN X-RAY MICROSCOPY AND SPATIALLY RESOLVED XANES SPECTROSCOPY OF ORGANIC SPECIMENS
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DOI：
10.1126/science.1439809
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发表时间：
1992-11-06
期刊：
影响因子：
56.9
通讯作者：
WILLIAMS, S
中科院分区：
文献类型：
--
作者：
ADE, H;ZHANG, X;WILLIAMS, S
在国家同步辐射光源的扫描透射X射线显微镜已被用来记录X射线吸收近边结构（XANES）光谱从0.01平方微米区域的有机样品。观察到的光谱特征反映了占主导地位的吸收原子的分子结构，并提供了高分辨率成像与化学灵敏度的对比机制。该技术与碳K吸收边附近的X射线能量一起使用，以识别和映射各种聚合物共混物中的分离相，并以55纳米的空间分辨率映射染色体中的DNA分布。
The scanning transmission x-ray microscope at the National Synchrotron Light Source has been used to record x-ray absorption near-edge structure (XANES) spectra from 0.01-square-micrometer regions of organic specimens. The spectral features observed reflect the molecular structure of the dominant absorbing atoms and provide the contrast mechanism for high-resolution imaging with chemical sensitivity. This technique was used with x-ray energies near the carbon K absorption edge to identify and map separate phases in various polymer blends and to map the DNA distribution in chromosomes with a spatial resolution of 55 nanometers.