Immuno-enhancement of Taishan Pinus massoniana pollen polysaccharides on recombinant Bordetella avium ompA expressed in Pichia pastoris
Immuno-enhancement of Taishan Pinus massoniana pollen polysaccharides on recombinant Bordetella avium ompA expressed in Pichia pastoris
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DOI：
10.1016/j.micpath.2016.03.002
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发表时间：
2016-06-01
影响因子：
3.8
通讯作者：
Zhu, Ruiliang
中科院分区：
文献类型：
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作者：
Liu, Liping;Yu, Cuilian;Zhu, Ruiliang
由禽波氏杆菌引起的波氏杆菌病一直是困扰我国养禽业的经济问题。缺乏具有良好保护能力的疫苗。因此，开发针对B的新型疫苗。禽流感是关键。在此，我们构建了能够表达B的外膜蛋白A（ompA）的重组毕赤酵母。目的：制备ompA亚单位重组疫苗，并评价其免疫效果。为进一步研究泰山马尾松花粉多糖（TPPPS）对ompA亚单位疫苗的免疫调节作用，分别以20、40和60 mg/mL的TPPPS作为ompA亚单位疫苗的佐剂。常规弗氏不完全佐剂作为TPPPS的对照。各组鸡分别免疫3次。监测期间检测血清抗体滴度、血清IL-4浓度、外周血CD 4（+）和CD 8（+）T淋巴细胞百分比、淋巴细胞转化率和保护率。结果表明，ompA纯疫苗可诱导小鼠产生抗ompA抗体，分泌IL-4，提高外周血CD 4 + T淋巴细胞计数和淋巴细胞转化率。此外，纯ompA疫苗在B之后提供了71.67%的保护率。avium挑战。TPPPS佐剂疫苗诱导的免疫应答水平高于纯ompA疫苗，其中60 mg/mL TPPPS佐剂疫苗的免疫效果最佳，保护率为91.67%。我们的研究结果表明，该重组B。鸡ompA亚单位疫苗与TPPPS联合应用具有较强的免疫刺激作用。结果为B提供了新的视角。禽亚单位疫苗研究。(C)2016爱思唯尔有限公司版权所有。
Bordetellosis, caused by Bordetella avium, continues to be an economic problem in the poultry industry of China. Vaccines with good protective ability are lacking. Thus, developing a novel vaccine against the B. avium infection is crucial. Here, we constructed a recombinant Pichia pastoris transformant capable of expressing the outer membrane protein A (ompA) of B. avium to prepare the recombinant ompA subunit vaccine and then evaluated its immune effects. To further investigate the immunomodulation effects of Taishan Pinus massoniana pollen polysaccharides (TPPPS) on this subunit vaccine, three concentrations (20, 40, and 60 mg/mL) of TPPPS were used as the adjuvants of the ompA subunit vaccine respectively. The conventional Freund's incomplete adjuvant served as the control of TPPPS. Chickens in different groups were separately vaccinated with these vaccines thrice. During the monitoring period, serum antibody titers, concentrations of serum IL-4, percentages of CD4(+) and CD8(+) T-lymphocytes in the peripheral blood, lymphocyte transformation rate, and protection rate were detected. Results showed that the pure ompA vaccine induced the production of anti-ompA antibody, the secretion of IL-4, the increase of CD4(+) T-lymphocytes counts and lymphocyte transformation rate in the peripheral blood. Moreover, the pure ompA vaccine provided a protection rate of 71.67% after the B. avium challenge. Notably, TPPPS adjuvant vaccines induced higher levels of immune responses than the pure ompA vaccine, and 60 mg/mL TPPPS adjuvant vaccine showed optimal immune effects and had a 91.67% protection rate. Our findings indicated that this recombinant B. avium ompA subunit vaccine combined with TPPPS had high immunostimulatory potential. Results provided a new perspective for B. avium subunit vaccine research. (C) 2016 Elsevier Ltd. All rights reserved.