Diverse dengue type 2 virus populations contain recombinant and both parental viruses in a single mosquito host
Diverse dengue type 2 virus populations contain recombinant and both parental viruses in a single mosquito host
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DOI：
10.1128/jvi.77.7.4463-4467.2003
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发表时间：
2003-04-01
影响因子：
5.4
通讯作者：
Aaskov, J
中科院分区：
文献类型：
--
作者：
Craig, S;Thu, HM;Aaskov, J
从埃及伊蚊个体的登革热 2 (DEN-2) 病毒群体和登革热患者血清中采集的包膜 (E) 蛋白基因被复制为 cDNA、克隆并测序。超过70%的克隆中E基因的核苷酸序列与相应病毒群体的共有序列有多达11个位点不同，并且94个克隆中的24个包含至少一个终止密码子。病毒种群的恢复间隔长达两年，产生了 E 基因具有相似多态性的克隆。对于一只蚊子，获得的克隆分为两种基因型。一组序列与1995年以来在同一地点（缅甸仰光）从登革热患者身上回收的病毒密切相关，被归类为亚洲1型基因型。第二组是大都会基因型病毒，2000年也在仰光流行，与1999年从中国南方采样的DEN-2病毒有关。最后，一个克隆被鉴定为由这两种“亲本”基因型的部分组成的重组基因组。这是重组和亲代登革热病毒在单一宿主中的首次报道。
Envelope (E) protein genes sampled from populations of dengue 2 (DEN-2) virus in individual Aedes aegypti mosquitoes and in serum from dengue patients were copied to cDNA, cloned, and sequenced. The nucleotide sequences of the E genes in more than 70% of the clones differed from the consensus sequence for the corresponding virus population at up to 11 sites, and 24 of the 94 clones contained at least one stop codon. Virus populations recovered up to 2 years apart yielded clones with similar polymorphisms in the E gene. For one mosquito, the clones obtained fell into two genotypes. One group of sequences was closely related to those of viruses recovered from dengue patients in the same locality (Yangon, Myanmar) since 1995 and were classified as Asian 1 genotype. The second group were Cosmopolitan genotype viruses which were also circulating in Yangon in 2000 and which were related to DEN-2 viruses sampled from southern China in 1999. Finally, one clone was, identified as a recombinant genome composed of portions of these two "parental" genotypes. This is the first report of recombinant and parental dengue viruses in a single host.