Estimation of skeletal muscle interstitial adenosine during forearm dynamic exercise in humans.
Estimation of skeletal muscle interstitial adenosine during forearm dynamic exercise in humans.
复制标题
人类前臂动态运动期间骨骼肌间质腺苷的估计。
DOI：
10.1161/01.hyp.35.5.1124
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Biaggioni,I
中科院分区：
文献类型：
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作者：
Costa,F;Heusinkveld,J;Ballog,R;Davis,S;Biaggioni,I
- 已经提出腺苷是一种代谢信号，其触发参与运动加压反射的肌肉传入的激活。 此外，外源性腺苷诱导交感神经激活，其模拟运动升压反射，并且腺苷受体的阻断抑制运动诱导的交感神经激活。因此，我们假设腺苷在运动过程中由肌肉局部释放。我们使用微透析探针，放置在屈趾浅肌，估计肌肉间质腺苷水平在人类。我们通过增加腺苷的浓度来确定透析液中产生的梯度，从而估计静息时体内肌肉间质腺苷浓度（0.292±0.058 μmol/L，n=4）。在50%最大随意收缩的间歇动力运动中，肌间质腺苷浓度从0.23±0.04 μmol/L增加到0.82±0.14 μmol/L（n=14，P<0.001）。 乳酸从0.8±0.1增加到2.3± 0.3mmol/L（P<0.001）。低强度（15%最大自主收缩）间歇动态运动使腺苷浓度从0.104±0.02 μmol/L增加到0.42±0.16 μmol/L（n=7）。与非缺血性运动（0.095 ± 0.02至0.25±0.12 μ mol/L）相比，在这种低水平运动中加入缺血性运动产生了更大的腺苷增加（从0.095 ± 0.02至0.48±0.2 μ mol/L）。这些结果表明，微透析是有用的，在估计腺苷浓度，并在反映肌肉间质腺苷在人体动态运动过程中的变化。
—It has been proposed that adenosine is a metabolic signal that triggers activation of muscle afferents involved in the exercise pressor reflex. Furthermore, exogenous adenosine induces sympathetic activation that mimics the exercise pressor reflex, and blockade of adenosine receptors inhibits sympathetic activation induced by exercise. Thus, we hypothesize that adenosine is released locally by the muscle during exercise. We used microdialysis probes, placed in the flexor digitorium superficialis muscle, to estimate muscle interstitial adenosine levels in humans. We estimated resting in vivo muscle interstitial adenosine concentrations (0.292±0.058 μmol/L, n=4) by perfusing increasing concentrations of adenosine to determine the gradient produced in the dialysate. Muscle interstitial adenosine concentrations increased from 0.23±0.04 to 0.82±0.14 μmol/L (n=14,P<0.001) during intermittent dynamic exercise at 50% of maximal voluntary contraction. Lactate increased from 0.8±0.1 to 2.3±0.3 mmol/L (P<0.001). Lower intensity (15% maximal voluntary contraction) intermittent dynamic exercise increased adenosine concentrations from 0.104±0.02 to 0.42±0.16 μmol/L (n=7). The addition of ischemia to this low level of exercise produced a greater increase in adenosine (from 0.095±0.02 to 0.48±0.2 μmol/L) compared with nonischemic exercise (0.095±0.02 to 0.25±0.12 μmol/L). These results indicate that microdialysis is useful in estimating adenosine concentrations and in reflecting changes in muscle interstitial adenosine during dynamic exercise in humans.