Excess antibody immunoassays for rat glandular kallikreins. Measurement of kallikrein from different organs in the presence of cross-reacting antigens.
Excess antibody immunoassays for rat glandular kallikreins. Measurement of kallikrein from different organs in the presence of cross-reacting antigens.
复制标题
大鼠腺激肽释放酶的过量抗体免疫测定。
DOI：
10.1016/0022-1759(83)90192-8
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发表时间：
1983
影响因子：
2.2
通讯作者：
M. Holck
中科院分区：
文献类型：
--
作者：
L. Johansen;T. Orstavik;K. Nustad;M. Holck
一种免疫放射测定法已被开发用于测量大鼠腺体钾激肽。本文对该方法的特异性和敏感性进行了进一步的研究。通过适当的抗体预吸收，克服了免疫交叉反应抗原的干扰问题。因此，建立了一种方法，即使在与小激肽共享免疫决定因素和底物特异性的酶存在的情况下，也可以测量免疫反应性小激肽的酶活性。已经评估了免疫放射测定法的两种变体。同时添加所有试剂的简化版本给出的结果等于在原始分析中获得的结果。通过延迟添加固相抗体的进一步修饰，可以显著提高测定灵敏度。
An immunoradiometric assay has previously been developed for measurement of rat glandular kallikrein. In the present paper, further studies on the specificity and sensitivity of the method are described. Problems of interference of immunologically cross-reacting antigens were overcome by proper preabsorption of the antibody. A method was thus established in which enzymatic activity of the immunoreactive kallikrein could be measured even in the presence of enzymes sharing immunological determinants and substrate specificity with kallikrein. Two variants of the immunoradiometric assay have been evaluated. A simplified version with simultaneous addition of all reagents gave results equal to those obtained in the original assay. A further modification with delayed addition of the solid-phase antibody, gave considerable improvement in assay sensitivity.