CLONALLY RELATED CORTICAL-CELLS SHOW SEVERAL MIGRATION PATTERNS
CLONALLY RELATED CORTICAL-CELLS SHOW SEVERAL MIGRATION PATTERNS
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DOI：
10.1126/science.3137660
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发表时间：
1988-09-09
期刊：
影响因子：
56.9
通讯作者：
CEPKO, CL
中科院分区：
文献类型：
--
作者：
WALSH, C;CEPKO, CL
哺乳动物的大脑皮层被组织成具有共同功能属性的细胞列。在胚胎发育期间，皮质神经元在靠近侧脑室的深处形成，并沿放射状迁移到它们的最终位置。这一观察结果表明，大脑皮质由克隆相关神经元的放射状个体发育单位组成。在本文报道的实验中，通过使用携带大肠杆菌β-半乳糖苷酶基因的逆转录病毒载体研究大鼠大脑皮质中的细胞谱系来验证这一假设，该基因很容易被可视化。标记的、克隆性相关的皮质神经元不出现在简单的柱状排列中。相反，克隆相关神经元进入了几个不同的放射柱，显然是通过沿不同的放射状神经胶质纤维迁移。
The mammalian cerebral cortex is organized into columns of cells with common functional properties. During embryogenesis, cortical neurons are formed deep, near the lateral ventricles, and migrate radially to their final position. This observation led to the suggestion that the cortex consists of radial, ontogenetic units of clonally related neurons. In the experiments reported here, this hypothesis was tested by studying cell lineage in the rat cortex with a retroviral vector carrying the Escherichia coli .beta.-galactosidase gene, which can be easily visualized. Labeled, clonally related cortical neurons did not occur in simple columnar arrays. Instead, clonally related neurons entered several different radial columns, apparently by migrating along different radial glial fibers.