Effects of phenolic acids on free radical scavenging and heavy metal bioavailability in kandelia obovata under cadmium and zinc stress
Effects of phenolic acids on free radical scavenging and heavy metal bioavailability in kandelia obovata under cadmium and zinc stress
复制标题
镉、锌胁迫下酚酸对秋茄自由基清除及重金属生物利用度的影响
DOI：
10.1016/j.chemosphere.2020.126341
复制
发表时间：
2020-06-01
期刊：
影响因子：
8.8
通讯作者：
Yan, Chongling
中科院分区：
文献类型：
--
作者：
Chen, Shan;Lin, Ruiyu;Yan, Chongling
目前植物对重金属的耐性机制研究没有考虑不同酚酸对重金属生物有效性的影响，以及与抗氧化物酶系统清除羟基自由基能力的比较。本研究通过在底泥中添加镉(Cd)和锌(Zn)的盆栽试验，研究了不同酚酸对倒伏秋茄体内Cd和Zn毒性的影响及其对羟基自由基的主要清除作用。结果表明，100 mg·kg~(-1)锌处理促进了高浓度镉、锌胁迫下植株的生长。在Cd和Zn胁迫下，倒卵叶草中酚酸的代谢主要通过苯丙烷和类黄酮类途径，辅以莽草酸和单酚醇途径。从该植物中检出11种具有不同清除自由基能力的酚酸，包括焦性没食子酸(Gal)、香豆酸(Cou)、原儿茶酸(Pro)、绿原酸(Chl)、4-羟基苯甲酸(Hyd)、咖啡酸(Caf)、香草酸(Van)、阿魏酸(Fer)、苯甲酸(Ben)和水杨酸(SAL)。通过在沉积物中添加酚酸，绿原酸(Chl)、焦性没食子酸(Gal)、肉桂酸(Cin)和香豆酸(CuU)对Cd或Zn转化为残留物的活性较强，绿原酸(Chl)、焦性没食子酸(Gal)、阿魏酸(Fer)和咖啡酸(Caf)清除羟基自由基的能力较强。综上所述，在高浓度Cd、Zn胁迫下，倒卵草易合成清除自由基能力强、改变Cd、Zn生物有效性的酚酸类物质。酚酸通过清除自由基，参与镉、锌的吸收和耐受，在缓解重金属胁迫中发挥重要作用。研究结果将为红树林湿地保护提供重要的理论依据和方法指导。(C)2020爱思唯尔有限公司。保留所有权利。
Current mechanism studies in plant heavy metal tolerance do not consider the effects of different phenolic acids on the bioavailability of heavy metals and the comparison with antioxidant enzyme system in the hydroxyl radical scavenging capacity. In present study, by a set of pot culture experiments with adding cadmium (Cd) and zinc (Zn) to the sediments, the effects of different phenolic acids on the toxicity of Cd and Zn in Kandelia obovata and the dominant role in scavenging hydroxyl radicals were evaluated. The results showed that 100 mg kg(-1) Zn treatment promoted the growth of plant under high concentrations of Cd and Zn stress. Under the stress of Cd and Zn, the phenolic acids were mainly metabolized by phenylpropanoid and flavonoid pathways, supplemented by shikimate and monolignol pathways in K. obovata. Eleven phenolic acids with different abilities of scavenging free radicals were detected in the plant, including pyrogallic acid (Gal), coumaric acid (Cou), protocatechuic acid (Pro), chlorogenic acid (Chl), 4-hydroxy benzoic acid (Hyd), caffeic acid (Caf), vanillic acid (Van), ferulic acid (Fer), benzoic acid (Ben), and salicylic acid (Sal). By adding phenolic acids to the sediments, chlorogenic acid (Chl), pyrogallic acid (Gal), cinnamic acid (Cin), and coumaric acid (Cou) behave as more reactive in changing Cd or Zn into residual fractions than the others, and chlorogenic acid (Chl), pyrogallic acid (Gal), ferulic acid (Fer) and caffeic acid (Caf) have higher ability of scavenging hydroxyl radicals than the others. In summary, K. obovata tends to synthesize phenolic acids with strong scavenging ability of free radicals and changing the bioavailability of Cd and Zn under high concentration of Cd and Zn stress. Phenolic acids played a crucial role in the mitigative effect of heavy metal stress via scavenging free radicals and involving in the process of Cd and Zn uptake and tolerance. The results will provide important theoretical basis and method guidance for mangrove wetland conservation. (C) 2020 Elsevier Ltd. All rights reserved.