Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.
Measuring beta-galactose exposure on platelets: Standardization and healthy reference values.
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DOI：
10.1002/rth2.12369
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发表时间：
2020-07
影响因子：
4.6
通讯作者：
Borgel D
中科院分区：
文献类型：
--
作者：
Lasne D;Pascreau T;Darame S;Bourrienne MC;Tournoux P;Philippe A;Ziachahabi S;Suarez F;Marcais A;Dupont A;Denis CV;Kauskot A;Borgel D
正确诊断血小板减少症的原因对于患者的适当管理至关重要。低唾液酸化/去唾液酸化（以异常高的β-半乳糖暴露为特征）加速血小板清除并可导致血小板减少症。然而，之前尚未定义健康个体中β-半乳糖暴露的参考范围。本研究的目的是开发一种用于临床实验室实施的血小板β-半乳糖暴露的标准化测定方法。使用流式细胞术和蓖麻凝集素（RCA）测定富血小板血浆中的β-半乳糖暴露量。招募了120名健康成年人来研究变异性。我们确定了最佳RCA浓度为12.5 μg/mL。该测量值可稳定长达4小时（平均荧光强度[MFI]-RCA：0小时为1233 ± 329，4小时为1480 ± 410）。血小板计数未诱导RCA变化，并且在采血后24小时内检测时RCA测量值保持稳定（MFI-RCA：第0天为1252 ± 434，采血后24小时为1140 ± 297）。考虑到血小板大小，结果应表示为RCA/前向散射比。我们使用该检测方法研究了120名健康成年人的变异性，发现该比率与性别和血型无关。我们在健康人群中定义了一个正常范围，并对几个分析前和分析变量以及阳性和阴性对照进行了评价。该测定可帮助诊断与β-半乳糖暴露变化相关的血小板减少性疾病。
Correct diagnosis of the cause of thrombocytopenia is crucial for the appropriate management of patients. Hyposialylation/desialylation (characterized by abnormally high β‐galactose exposure) accelerates platelet clearance and can lead to thrombocytopenia. However, the reference range for β‐galactose exposure in healthy individuals has not been defined previously. The objective of the present study was to develop a standardized assay of platelet β‐galactose exposure for implementation in a clinical laboratory. β‐Galactose exposure was measured in platelet‐rich plasma by using flow cytometry and Ricinus communis agglutinin (RCA). A population of 120 healthy adults was recruited to study variability. We determined an optimal RCA concentration of 12.5 μg/mL. The measure was stable for up to 4 hours (mean fluorescence intensity [MFI]‐RCA: 1233 ± 329 at 0 hour and 1480 ± 410 at 4 hours). The platelet count did not induce a variation of RCA and the measure of RCA was stable when tested up to 24 hours after blood collection (MFI‐RCA: 1252 ± 434 at day 0 and 1140 ± 297 24 hours after blood sampling). To take into account the platelet size, results should be expressed as RCA/forward scatter ratio. We used the assay to study variability in 120 healthy adults, and we found that the ratio is independent of sex and blood group. We defined a normal range in a healthy population and several preanalytical and analytical variables were evaluated, together with positive and negative controls. This assay may assist in the diagnosis of thrombocytopenic diseases linked to changes in β‐galactose exposure.
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影响因子：
10.1
作者：
Marini I;Zlamal J;Faul C;Holzer U;Hammer S;Pelzl L;Bethge W;Althaus K;Bakchoul T
通讯作者：
Bakchoul T
影响因子：
16.6
作者：
Li J;van der Wal DE;Zhu G;Xu M;Yougbare I;Ma L;Vadasz B;Carrim N;Grozovsky R;Ruan M;Zhu L;Zeng Q;Tao L;Zhai ZM;Peng J;Hou M;Leytin V;Freedman J;Hoffmeister KM;Ni H
通讯作者：
Ni H
影响因子：
82.9
作者：
通讯作者：
--
影响因子：
9.7
作者：
Arnold, Suzanne V.;Jones, Philip G.;Spertus, John A.
通讯作者：
Spertus, John A.
影响因子：
10.1
作者：
Dupont, Annabelle;Soukaseum, Christelle;Kauskot, Alexandre
通讯作者：
Kauskot, Alexandre