Establishment and characterization of nurse cell-like clones from human skin. Nurse cell-like clones can stimulate autologous mixed lymphocyte reaction.
Establishment and characterization of nurse cell-like clones from human skin. Nurse cell-like clones can stimulate autologous mixed lymphocyte reaction.
复制标题
人类皮肤护理细胞样克隆的建立和表征。
作者：
S. Iwagami;S. Furue;T. Toyosaki;Tatsuya Horikawa;Hideyuki Doi;Susumu Satomi;Tsunetoshi Itoh;Tsuneaki Sakata;Ryuji Suzuki
我们已经从人类皮肤的长期培养中建立了类似护士细胞的克隆体。这些人类皮肤护理细胞（HSNC）样克隆是I型胶原+、IV型胶原-、vimentin+、细胞角蛋白-、CD44+、CD54+，并且VCAM-1弱阳性，并且很容易被允许T淋巴细胞迁移到HSNC下的假上皮细胞识别。HSNCs与各种T细胞系在吊滴培养体系中形成典型的复合体。大多数人类和小鼠的T细胞，以及除T细胞以外的一些肿瘤细胞系，包括B淋巴瘤和髓母细胞瘤细胞，在HSNC克隆下迁移。HSNC克隆产生多种细胞因子，包括IL-6、IL-7、IL-8、IL-9、粒细胞CSF （G-CSF）、粒细胞-巨噬细胞CSF （GM-CSF）、巨噬细胞CSF （CSF-1）、tgf - β 1和c-kit配体，但不能产生IL-1 α、IL-1 β、IL-2、IL-3、IL-4、tnf - α和tnf - β。这些特征与从小鼠胸腺中培养的乳母细胞相似。此外，ifn - γ预处理的表达MHC II类Ags的HSNC克隆在自体PBMCs中诱导了自体混合淋巴细胞反应（AMLR）增殖，并对改变的自体细胞和各种肿瘤细胞表现出细胞毒性。提示HSNCs在皮肤组织免疫调节中发挥重要作用。
We have established nurse cell-like clones from long-term cultures of the human skin. These human skin nurse cell (HSNC)-like clones were type I collagen+, type IV collagen-, vimentin+, cytokeratin-, CD44+, CD54+, and weakly positive for VCAM-1, and easily identified by the pseudoemperipolesis that allowed T lymphocytes to migrate beneath the HSNCs. HSNCs and various T cell lines formed a typical complex in the hanging drop culture system. The majority of human and murine T cells, and some of the tumor cell lines other than T cells, including B lymphoma and myeloblastoma cells, migrated beneath the HSNC clones. HSNC clones produced various cytokines, including IL-6, IL-7, IL-8, IL-9, granulocyte CSF (G-CSF), granulocyte-macrophage CSF (GM-CSF), macrophage CSF (CSF-1), TGF-beta 1, and c-kit ligand, but could not produce IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, TNF-alpha, or TNF-beta. These characteristics were similar to those of nurse cells established from the murine thymus. Furthermore, IFN-gamma-pretreated HSNC clones that expressed MHC class II Ags induced autologous mixed lymphocyte reaction (AMLR) in autologous PBMCs to proliferate and exhibit the cytotoxicity against altered autologous cells and various tumor cells. These results suggest that HSNCs play an important role in the immunoregulation at skin tissues.