The Ld Cht1 gene encodes the secretory chitinase of the human pathogen Leishmania donovani.
The Ld Cht1 gene encodes the secretory chitinase of the human pathogen Leishmania donovani.
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Ld Cht1 基因编码人类病原体杜氏利什曼原虫的分泌性几丁质酶。
DOI：
10.1016/s0378-1119(98)00011-0
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发表时间：
1998
期刊：
影响因子：
3.5
通讯作者：
D. Dwyer
中科院分区：
文献类型：
--
作者：
A. Shakarian;D. Dwyer
杜氏利什曼原虫前鞭毛体在体外生长过程中会释放几丁质酶活性。基于 PCR 的策略，鉴定出能够编码杜氏乳杆菌几丁质酶（Ld Cht1，1374bp）的单拷贝 ORF。通过逆转录和 PCR 扩增，Ld Cht1 被杜氏乳杆菌前鞭毛体主动转录。推导的 Ld Cht1 氨基酸序列显示出对已知几丁质酶的高度保守性，包括假定的活性和两个底物结合位点。针对衍生自其推导的氨基酸序列的四种肽产生的抗血清免疫沉淀了大约 10%。 Ld Cht1 的 50-kDa 体外转录/翻译产物。此外，该抗血清还可以免疫沉淀天然杜氏乳杆菌 50-kDa Cht1 蛋白以及这些寄生虫合成和释放的天然几丁质酶活性。总的来说，这些数据表明 Ld Cht1 编码这种重要的人类病原体的几丁质酶。
Leishmania donovani promastigotes were shown to release chitinase activity during their growth in vitro. A PCR-based strategy, identified a single copy ORF capable of encoding the L. donovani chitinase (Ld Cht1, 1374bp). Ld Cht1 was shown to be actively transcribed by L. donovani promastigotes using reverse transcription and PCR amplification. The deduced aa sequence of Ld Cht1 showed high conservation to known chitinases including the putative active and two substrate binding sites. Antiserum generated against four peptides derived from its deduced aa sequence immunoprecipitated an approx. 50-kDa in vitro transcription/translation product of Ld Cht1. Further, this antiserum also immunoprecipitated both the native L. donovani 50-kDa Cht1 protein and the native chitinase activity synthesized and released by these parasites. Cumulatively, these data demonstrated that Ld Cht1 encoded the chitinase of this important human pathogen.
影响因子：
1.5
作者：
delaVega, H;Specht, CA;Samuelson, J
通讯作者：
Samuelson, J
褶皱链霉菌几丁质酶 (chitinase-63) 在大肠杆菌中的克隆和表达。
DOI：
--
发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Robbins,PW;Albright,C;Benfield,B
通讯作者：
Benfield,B