STREPTOTHRICIN-F, AN INHIBITOR OF PROTEIN-SYNTHESIS WITH MISCODING ACTIVITY
STREPTOTHRICIN-F, AN INHIBITOR OF PROTEIN-SYNTHESIS WITH MISCODING ACTIVITY
复制标题
DOI：
10.7164/antibiotics.31.1137
复制
发表时间：
1978-01-01
影响因子：
3.3
通讯作者：
THRUM, H
中科院分区：
文献类型：
--
作者：
HAUPT, I;HUBENER, R;THRUM, H
研究了链丝菌素F对大肠杆菌完整细胞内大分子合成和游离蛋白质合成的影响。蛋白质合成显然是链丝菌素F抑制生长的E. coli细胞。利用E.对poly（U）介导的大肠杆菌多肽合成有抑制作用，而对poly（A）和poly（C）介导的多肽合成有促进作用。链丝菌素F引起了对大肠杆菌中poly（U）、poly（A）和poly（C）蛋白合成的翻译的误读。coli系统。链丝菌素F的误读程度随着药物浓度的增加而增加。结果与其他错误编码的抗生素进行了比较。在大鼠肝脏提取物中，由poly（U）或内源性mRNA指导的蛋白质合成不受抑制。
The effect of streptothricin F on macromolecular syntheses in intact cells and cell-free protein synthesis of Escherichia coli was studied. Protein synthesis was apparently the primary site of inhibition by streptothricin F in growing E. coli cells. Cell-free polypeptide synthesis from E. coli directed by poly (U) was inhibited, while poly (A) and poly (C) directed polypeptide syntheses were stimulated by the drug. Streptothricin F caused misreading of translation of poly (U), poly (A) and poly (C) directed protein syntheses in E. coli systems. The extent of misreading by streptothricin F increases with increasing drug concentrations. The results are compared with those of other miscoding antibiotics. In rat liver extracts protein synthesis directed by poly (U) or endogenous mRNA was not inhibited.