Gas chromatographic determination of 3-butene-1,2-diol in urine samples after 1,3-butadiene exposure
Gas chromatographic determination of 3-butene-1,2-diol in urine samples after 1,3-butadiene exposure
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DOI：
10.1016/s0378-4347(99)00227-3
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发表时间：
1999-07-09
期刊：
影响因子：
--
通讯作者：
Peltonen, K
中科院分区：
文献类型：
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作者：
Anninen-Klemetti, T;Vaaranrinta, R;Peltonen, K
1，3-丁二醇是一种重要的工业化学品，也是一种常见的环境污染物。由于其疑似致癌性，丁二烯相关研究已获得高度活跃。到目前为止，明显缺乏知识的是，不存在可以从体液或排泄物中检测至少一种丁二烯代谢物的生物监测方法。在本通信中，我们描述了一个强大的和简单的分析方法，可用于生物监测的目的。本文建立了一种测定吸入不同浓度1，3-丁二烯大鼠尿样中3-丁烯-1，2-二醇的方法。该方法基于液-液萃取和随后的气相色谱分析。浓度为2.2 μ g/ml的3-丁烯-1，2-二醇的萃取效率为95%（SD=+/-3%，n=3），并且通过使用氯化钠饱和和异丙醇作为萃取溶剂来实现。气相色谱分析的标准偏差为+/-2%（n=12），检测限为0.08 μ g/ml，定量限为0.11 μ g/ml（SD=+/-4.8%，n=3），分析在0.11 - 486 μ g/ml范围内呈线性（R=0.9987）。暴露于1，3-丁二烯的动物显示，作为丁二烯暴露的函数，3-丁烯-1，2-二醇线性排泄到尿液中。在接触过程中，在所用的任何接触水平下均未观察到1，3-丁二烯或3-丁烯-1，2-二醇进入体内的代谢饱和或蓄积。(C)1999 Elsevier Science B. V.保留所有权利。
1,3-Butadiene is an important industrial chemical and a common environmental contaminant. Because of its suspected carcinogenicity butadiene-related research has gained high activity. The obvious lack of knowledge so far has been that a biomonitoring method that can detect at least one of the metabolites of butadiene from body fluids or excretas does not exist. In this communication we describe a robust and simple analytical method which can be applied for biomonitoring purposes. We have developed a method that can detect 3-butene-1,2-diol in urine samples of rats inhalation-exposed to various concentrations of 1,3-butadiene. The method is based on liquid-liquid extraction and subsequent gas chromatographic analysis. The extraction efficiency of 3-butene-1,2-diol at a concentration of 2.2 mu g/ml was 95% (SD=+/-3%, n=3) and was achieved by using sodium chloride saturation and isopropanol as an extracting solvent. The standard deviation of the gas chromatographic analysis was +/-2% (n=12), the limit of detection was 0.08 mu g/ml, the limit of quantitation was 0.11 mu g/ml (SD=+/-4.8%, n=3) and the analysis was observed to be linear from 0.11 to 486 mu g/ml (R=0.9987). Animals exposed to 1,3-butadiene showed a linear excretion of 3-butene-1,2-diol into urine as a function of butadiene exposure. During the exposure saturation of metabolism or accumulation of 1,3-butadiene or 3-butene-1,2-diol into the body was not observed in any exposure levels used. (C) 1999 Elsevier Science B.V. All rights reserved.