Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits
Effects of Ultra-Purified Alginate Gel Implantation on Meniscal Defects in Rabbits
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DOI：
10.1177/0363546518816690
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发表时间：
2019-03-01
影响因子：
4.8
通讯作者：
Iwasaki, Norimasa
中科院分区：
文献类型：
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作者：
Kim, WooYoung;Onodera, Tomohiro;Iwasaki, Norimasa
背景：许多组织工程方法已经被研究，但其效用仍不清楚。假设：低内毒素、超纯藻酸盐（UPAL）无细胞凝胶植入兔桡骨缺损处可诱导纤维软骨再生。研究设计：对照实验室研究。方法：42只成年日本白色家兔分为2组，每组21只。在每只动物中，使用活检打孔器在内侧半月板的前角上创建直径为2 mm的圆柱形缺损。在对照组中，不对左侧内侧髋臼缺损进行治疗。在UPAL凝胶组中，将UPAL凝胶注入右侧内侧髋臼缺损，并通过CaCl 2溶液进行凝胶化。在术后3、6和12周评价样本。对于生物力学评价，使用来自完整动物的另外6个样本进行比较。结果如下：在3周时，UPAL凝胶组的宏观评分显著高于对照组（平均值+/- SE：5.6 +/- 0.82 vs 3.4 +/- 0.83，P = 0.010）、6周（5.9 +/- 0.72 vs 2.5 +/- 0.75，P = 0.026）和12周（5.2 +/- 1.21 vs 1.0 +/- 0.63，P = 0.020）。UPAL组在3周（2.1 +/- 0.31 vs 1.2 +/- 0.25，P = 0.029）和12周（2.2 +/- 0.55 vs 0.3 +/- 0.21，P = 0.016）时的组织学评分显著高于对照组。在第6周（24.325 +/- 3.920 N/mm vs 8.723 +/- 1.190 N/mm，P = 0.006）和第12周（27.804 +/- 6.169 N/mm vs不适用[由于破裂]），UPAL凝胶组修复组织的平均硬度显著高于对照组。结论：UPAL凝胶可促进纤维软骨组织对兔桡骨圆柱形缺损的自发修复。
Background: Many tissue-engineered methods for meniscal repair have been studied, but their utility remains unclear. Hypothesis: Implantation of low-endotoxin, ultra-purified alginate (UPAL) gel without cells could induce fibrocartilage regeneration on meniscal defects in rabbits. Study Design: Controlled laboratory study. Methods: Forty-two mature Japanese White rabbits were divided into 2 groups of 21 animals each. In each animal, a cylindrical defect measuring 2 mm in diameter was created with a biopsy punch on the anterior horn of the medial meniscus. In the control group, no treatment was applied on the left medial meniscal defect. In the UPAL gel group, the right medial meniscal defect was injected with the UPAL gel and gelated by a CaCl2 solution. Samples were evaluated at 3, 6, and 12 weeks postoperatively. For biomechanical evaluation, 6 additional samples from intact animals were used for comparison. Results: The macroscopic score was significantly greater in the UPAL gel group than in the control group at 3 weeks (mean +/- SE: 5.6 +/- 0.82 vs 3.4 +/- 0.83, P = .010), 6 weeks (5.9 +/- 0.72 vs 2.5 +/- 0.75, P = .026), and 12 weeks (5.2 +/- 1.21 vs 1.0 +/- 0.63, P = .020). The histological score was significantly greater in the UPAL group than in the control group at 3 weeks (2.1 +/- 0.31 vs 1.2 +/- 0.25, P = .029) and 12 weeks (2.2 +/- 0.55 vs 0.3 +/- 0.21, P = .016). The mean stiffness of the reparative tissue in the UPAL gel group was significantly greater than that in the control group at 6 weeks (24.325 +/- 3.920 N/mm vs 8.723 +/- 1.190 N/mm, P = .006) and at 12 weeks (27.804 +/- 6.169 N/mm vs not applicable [because of rupture]). Conclusion: The UPAL gel enhanced the spontaneous repair of fibrocartilage tissues in a cylindrical meniscal defect in rabbits.