Water soluble cryptophanes showing unprecedented affinity for xenon: Candidates as NMR-based biosensors
Water soluble cryptophanes showing unprecedented affinity for xenon: Candidates as NMR-based biosensors
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DOI：
10.1021/ja060266r
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发表时间：
2006-05-10
影响因子：
15
通讯作者：
Berthault, Patrick
中科院分区：
文献类型：
--
作者：
Huber, Gaspard;Brotin, Thierry;Berthault, Patrick
含OCH_2COOH取代甲氧基的Cryptophane是一类在生物pH条件下可溶于水的新型氙载体分子。本文利用H-1和NMR-129（热-和激光-极化溶解气体）研究了这类主体分子的结构和动力学行为以及它们与氙的相互作用。它们以不同的构象存在于水溶液中，交换速度很慢。这些构象之一的鞍形可以解释H-1 NMR谱。虽然处于这种构象的隐黄素无法与氙复合，但在另一种典型的冠-冠构象中发现了前所未有的高结合常数。因此，这些宿主分子可能是使用MRI-129进行生物传感的有价值的候选者。
Cryptophanes bearing OCH2COOH groups in place of the methoxy groups represent a new class of xenon-carrier molecules soluble in water at biological pH. By using H-1 and Xe-129 NMR (thermally-and laser-polarized dissolved gas), the structural and dynamical behaviors of these host molecules as well as their interaction with xenon are studied. They are shown to exist in aqueous solution under different conformations in very slow exchange. A saddle form present for one of these conformations could explain the H-1 NMR spectra. Whereas the cryptophanes in such a conformation are unable to complex xenon, unprecedented high binding constants are found for cryptophanes in the other canonical crown-crown conformation. These host molecules could therefore be valuable candidates for biosensing using Xe-129 MRI.