Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust.
Brachypodium distachyon T-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust.
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二穗短柄草 T-DNA 插入系：研究小麦条锈病非寄主抗性的模型病理系统
DOI：
10.1038/srep25510
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发表时间：
2016-05-03
影响因子：
4.6
通讯作者：
Qi X
中科院分区：
文献类型：
--
作者：
An T;Cai Y;Zhao S;Zhou J;Song B;Bux H;Qi X
小麦条锈病，由条锈菌 f 引起。 sp。小麦 (PST) 是最具破坏性的疾病之一，可在世界许多地区造成严重的产量损失。由于小麦基因组庞大且复杂，研究小麦与PST相互作用的分子机制十分困难。二穗短柄草已成为温带禾本科植物功能基因组学研究的模型系统。表型评估表明，短柄草对 PST 的反应是非宿主抗性（NHR），这使我们能够将这种植物病原体系统作为模型来探索小麦和 PST 的免疫反应和分子机制。在这里，我们报道了基于高效农杆菌介导的转化系统产生了约 7,000 个 T-DNA 插入系。获得了数百个比野生型 Bd21 更易感或更抗 PST 的突变体。三个T-DNA插入突变体的三个推定靶基因Bradi5g17540、BdMYB102和Bradi5g11590可能参与短柄草向小麦条锈病的NHR。这种T-DNA突变体的系统病理学研究将拓宽我们对NHR的认识，并有助于培育具有持久抗性的小麦品种。
Wheat stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most destructive diseases and can cause severe yield losses in many regions of the world. Because of the large size and complexity of wheat genome, it is difficult to study the molecular mechanism of interaction between wheat and PST. Brachypodium distachyon has become a model system for temperate grasses’ functional genomics research. The phenotypic evaluation showed that the response of Brachypodium distachyon to PST was nonhost resistance (NHR), which allowed us to present this plant-pathogen system as a model to explore the immune response and the molecular mechanism underlying wheat and PST. Here we reported the generation of about 7,000 T-DNA insertion lines based on a highly efficient Agrobacterium-mediated transformation system. Hundreds of mutants either more susceptible or more resistant to PST than that of the wild type Bd21 were obtained. The three putative target genes, Bradi5g17540, BdMYB102 and Bradi5g11590, of three T-DNA insertion mutants could be involved in NHR of Brachypodium distachyon to wheat stripe rust. The systemic pathologic study of this T-DNA mutants would broaden our knowledge of NHR, and assist in breeding wheat cultivars with durable resistance.