Monoclonal antibodies as probes of the topological arrangement of the alpha subunits of Escherichia coli RNA polymerase.
Monoclonal antibodies as probes of the topological arrangement of the alpha subunits of Escherichia coli RNA polymerase.
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单克隆抗体作为大肠杆菌 RNA 聚合酶 α 亚基拓扑排列的探针。
DOI：
10.1021/bi00434a027
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Krakow,JS
中科院分区：
文献类型：
--
作者：
Riftina,F;DeFalco,E;Krakow,JS
用三种单抗研究了大肠杆菌RNA聚合酶α亚基的性质。这些单抗均不能抑制d(AT)“定向合成r(AU)”。MAb129C4或mAb126C6可阻断RNA聚合酶核心的重组，而mAb124D1则无此作用。MAb129C4部分抑制早熟核心向成熟核心的转化，mAb126C6几乎完全抑制成熟核心的转化。这些数据表明，在核心组装过程中，至少有一个α亚基发生了构象变化。MAb126C6对组装的a的亲和力比对游离的a的亲和力增加，这也表明a在RNA聚合酶组装过程中经历了构象变化。双抗体结合研究表明，mAb124D1和mAb129C4的表位仅存在于RNA聚合酶的一个a亚基上。RNA聚合酶中a亚基中的一个亚基上的相关区域似乎暴露得很好，而第二个a亚基上的这个区域被与大的？和？‘亚单位区域的相互作用所屏蔽。单抗126C6表位所在的a区不受RNA聚合酶中亚基相互作用的阻碍。这些数据还表明，在全酶中，亚基可能靠近a亚基之一，可能靠近暴露程度更高的a亚基。由于a亚基中的一个亚基在与所研究的任何单抗结合后从2？复合体中解离出来，因此？这些研究表明，聚合酶核心的体外组装可能是通过形成更稳定的？复合体，然后添加第二个a亚基进行的。
Three monoclonal anti-antibodies were used tostudy the properties of the a subunit of Escherichia coliRNA polymerase. None of the monoclonal antibodies inhibited the d (AT)„-directed synthesis of r (AU)„. Reassembly of the RNA polymerase core was blocked by mAb 129C4 or mAb 126C6 while no effect was observed with mAb 124D1. The conversion of premature to mature core was partially inhibited by mAb 129C4 and almost totally inhibited by mAb 126C6. The data suggest that during the course of core assembly at least one of the a subunits undergoes conformational changes. The increase in affinity of mAb 126C6 for assembled a compared with free a also implies that a undergoes conformational changes during RNA polymerase assembly. Double antibody binding studies showed that the epitopes for mAb 124D1 and mAb 129C4 are available on only one of the a subunits in RNA polymerase. It would appear that the relevant domain on one of the a subunits in RNA polymerase is well exposed whereasthis domain on the second a subunit is shielded by interaction with regions of the large ß and ß'subunits. The a domain in which the epitope for mAb 126C6 resides is not impeded by subunit interactions in the RNA polymerase. The data obtained also suggest that in the holoenzyme the subunit may be positioned close to one of the a subunits, probably to the more exposed a. The ß complex is the minimal stable subassembly since one of the a subunits dissociates from the 2ß complex following binding of any of the monoclonal antibodies studied. These studies suggest that the in vitro assembly of the polymerase core may proceed via the formation of the more stable ß complex followed by addition of the second a subunit.
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抗 β 和抗 β 单克隆抗体对大肠杆菌 RNA 聚合酶活性影响的表征。
影响因子：
2.9
作者：
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DOI：
10.1111/j.1432-1033.1976.tb10286.x
发表时间：
1976
期刊：
European journal of biochemistry
影响因子：
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作者：
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影响因子：
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作者：
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影响因子：
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作者：
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DOI：
10.1111/j.1432-1033.1980.tb07221.x
发表时间：
1980
期刊：
European journal of biochemistry
影响因子：
--
作者：
P. Stöckel;R. May;I. Strell;Z. Cejka;W. Hoppe;H. Heumann;W. Zillig;H. Crespi
通讯作者：
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