Neovascularization in a mouse model via stem cells derived from human fetal amniotic membranes
Neovascularization in a mouse model via stem cells derived from human fetal amniotic membranes
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DOI：
10.1007/s00380-010-0064-6
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发表时间：
2011-03-01
影响因子：
1.5
通讯作者：
Choi, Ook Hwan
中科院分区：
文献类型：
--
作者：
Kim, Hwi Gon;Choi, Ook Hwan
在这项研究中，我们在免疫缺陷小鼠肢体缺血动物模型中评估了来自羊膜的培养扩增间充质干细胞（MSCs）对新生血管和血流的影响。采用胶原酶消化法培养人羊膜间充质干细胞。将人羊膜间充质干细胞（hAMSCs）肌注于已结扎股血管的缺血腿的三个不同部位。培养4周后，汇合后从人羊膜中分离出一群均匀的间充质细胞。我们进行了三组不同的小鼠模型[对照组，hAMSCs， hAMSCs的条件培养基（hAMSCs- cm）]。hamsc移植组后肢缺血模型血流恢复明显高于对照组。此外，hAMSCs-CM显著改善皮肤血流量。组织学检查显示，移植2周后肌纤维间质组织中检测到红色荧光（CM-DiI）标记的hAMSCs。本研究结果表明，hAMSCs可能是基础研究和临床应用中有吸引力的祖细胞或干细胞的替代来源。
In this study, we evaluated the effect of culture-expanded mesenchymal stem cells (MSCs), derived from amniotic membranes, on neovascularization and blood flow, in an animal model of limb ischemia in immune-deficient mice. MSCs were cultured from human amniotic membranes by collagenase digestion. Human amniotic mesenchymal stem cells (hAMSCs) were administered intramuscularly at three different sites of the ischemic leg whose femoral vessels were ligated. After 4 weeks of culture, a population of homogeneous mesenchymal cells was isolated from the human amniotic membranes after confluence was reached. We performed three different groups of mice model [controls, hAMSCs, conditioned media from the hAMSCs (hAMSCs-CM)]. The blood flow recovery in the hindlimb ischemia model was significantly higher in the hAMSC-transplanted group than in the control group. Moreover, hAMSCs-CM significantly improved the cutaneous blood flow. The histological examination showed that red fluorescence (CM-DiI)-labeled hAMSCs was detected in the interstitial tissues between the muscle fibers 2 weeks after transplantation. The results of this study showed that hAMSCs may be an attractive, alternative source of progenitor or stem cells for basic research as well as clinical applications.