Effects of exposure to an extremely low frequency electromagnetic field on hippocampal long-term potentiation in rat
Effects of exposure to an extremely low frequency electromagnetic field on hippocampal long-term potentiation in rat
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DOI：
10.1016/j.brainres.2014.03.041
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发表时间：
2014-05-20
期刊：
影响因子：
2.9
通讯作者：
Sarihi, Abdolrahman
中科院分区：
文献类型：
--
作者：
Komaki, Alireza;Khalili, Afshin;Sarihi, Abdolrahman
现代生活方式使几乎所有人都暴露在电磁场中，尤其是极低频电磁场(ELF-EMF)。长期暴露在ELF-EMEs中会导致神经元活动的持续性变化。然而，ELF-EMF在体内对突触效率的调节仍不清楚。在本研究中，我们研究了ELF-EMF是否可以改变大鼠海马区长时程增强(LTP)和成对脉冲比(PPR)的诱导。成年雄性Wistar大鼠29只，随机分为3组(ELF-EMF暴露组、假手术组和对照组)。ELF-EMF组连续暴露在磁场中90天(2小时/天)。自旋电磁场由圆形线圈(50赫兹，100微特斯拉)产生。假暴露的对照组被放在一个相同的房间里，没有电磁场。此后，用乌拉坦(2.0 mg/kg)深度麻醉大鼠，然后在穿孔通路(PP)和齿状回(DG)分别植入双极刺激和记录电极。高频刺激(HFS)诱导海马区LTP。长期暴露于ELF-EMF可增加LT-P的诱导。ELF-EMF组的EPSP斜率和PS波幅与其他组比较差异有统计学意义。总之，我们的数据表明，暴露在极低频电磁场下，PP-DG突触产生的突触可塑性发生了显著变化。ELF-EMF组HFS前后PPR无明显差异，提示LTP存在突触后表达部位。(C)2014爱思唯尔B.V.保留所有权利。
Modem lifestyle exposes nearly all humans to electromagnetic fields, particularly to extremely low frequency electromagnetic fields (ELF-EMFs). Prolonged exposure to ELF-EMEs induces persistent changes in neuronal activity. However, the modulation of synaptic efficiency by ELF-EMFs in vivo is still unclear. In the present study, we investigated whether ELF-EMFs can change induction of long-term potentiation (LTP) and paired-pulse ratio (PPR) in the rat hippocampal area. Twenty-nine adult male Wistar rats were divided into 3 groups (ELF-EMF exposed, sham and control groups). The ELF-EMF group was exposed to a magnetic field for 90 consecutive days (2 h/day). ELF-EMFs were produced by a circular coil (50 Hz, 100 micro Tesla). The sham-exposed controls were placed in an identical chamber with no electromagnetic field. After this period, rats were deeply anesthetized with urethane (2.0 mg/kg) and then a bipolar stimulating and recording electrode was implanted into the perforant pathway (PP) and dentate gyrus (DG), respectively. LTP in hippocampal area was induced by high-frequency stimulation (HFS). Prolonged exposure to ELF-EMFs increased LT-P induction. There was a significant difference in the slope of EPSP and amplitude of PS between the ELF-EMF group and other groups. In conclusion, our data suggest that exposure to ELF-EMFs produces a marked change in the synaptic plasticity generated in synapses of the PP-DG. No significant difference in PPR of ELF-EMF group before and after HFS suggests a postsynaptic expression site of LTP. (C) 2014 Elsevier B.V. All rights reserved.