Retinal Oxygen Metabolism During Normoxia and Hyperoxia in Healthy Subjects
Retinal Oxygen Metabolism During Normoxia and Hyperoxia in Healthy Subjects
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DOI：
10.1167/iovs.14-14593
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发表时间：
2014-08-01
影响因子：
4.4
通讯作者：
Schmetterer, Leopold
中科院分区：
文献类型：
--
作者：
Palkovits, Stefan;Lasta, Michael;Schmetterer, Leopold
目的。研究健康受试者在常氧和高氧状态下视网膜代谢的特征。本研究纳入46名健康受试者，可评价41名受试者的资料。使用动态血管分析仪测量视网膜血管直径，以及动脉和静脉的氧饱和度。此外，采用双向激光多普勒测速仪测量视网膜静脉血速度，计算视网膜血流量，并测量耳垂动脉化毛细血管血的氧分压和二氧化碳分压。在正常缺氧和100%氧气呼吸时进行测量。全体性高氧导致视网膜静脉直径（-13.0% +/- 4.5%）和动脉直径（-12.1% +/- 4.0%）、视网膜血流速（-43.4% +/- 7.7%）和视网膜血流量（-57.0% +/- 5.7%）显著降低（P均< 0.001）。视网膜动脉氧饱和度升高（+4.4% +/- 2.3%），视网膜静脉氧饱和度升高（+19.6% +/- 6.2%），动静脉氧含量差异显著降低（-29.4% +/- 19.5%）（P < 0.001）。动脉血血氧压由90.2 +/- 7.7显著升高至371.3 +/- 92.7 mm Hg （P < 0.001）。计算眼内吸氧量减少62.5% +/- 9.5% （P < 0.001）。我们的数据与之前的动物数据所支持的假设相一致，即在100%氧气呼吸过程中，视网膜内部消耗的大量氧气来自脉络膜。在不量化血流的情况下解释视网膜动脉和静脉的氧饱和度数据是困难的。
PURPOSE. To characterize retinal metabolism during normoxia and hyperoxia in healthy subjects.METHODS. Forty-six healthy subjects were included in the present study, and data of 41 subjects could be evaluated. Retinal vessel diameters, as well as oxygen saturation in arteries and veins, were measured using the Dynamic Vessel Analyzer. In addition, retinal venous blood velocity was measured using bidirectional laser Doppler velocimetry, retinal blood flow was calculated, and oxygen and carbon dioxide partial pressures were measured from arterialized capillary blood from the earlobe. Measurements were done during normoxia and during 100% oxygen breathing.RESULTS. Systemic hyperoxia caused a significant decrease in retinal venous diameter (-13.0% +/- 4.5%) and arterial diameter (-12.1% +/- 4.0%), in retinal blood velocity (-43.4% +/- 7.7%), and in retinal blood flow (-57.0% +/- 5.7%) (P < 0.001 for all). Oxygen saturation increased in retinal arteries (+4.4% +/- 2.3%) and in retinal veins (+19.6% +/- 6.2%), but the arteriovenous oxygen content difference significantly decreased (-29.4% +/- 19.5%) (P < 0.001 for all). Blood oxygen tension in arterialized blood showed a pronounced increase from 90.2 +/- 7.7 to 371.3 +/- 92.7 mm Hg (P < 0.001). Calculated oxygen extraction in the eye decreased by as much as 62.5% +/- 9.5% (P < 0.001).CONCLUSIONS. Our data are compatible with the hypothesis that during 100% oxygen breathing a large amount of oxygen, consumed by the inner retina, comes from the choroid, which is supported by previous animal data. Interpretation of oxygen saturation data in retinal arteries and veins without quantifying blood flow is difficult.