Multispectral optical imaging device for in vivo detection of oral neoplasia
Multispectral optical imaging device for in vivo detection of oral neoplasia
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DOI：
10.1117/1.2904658
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发表时间：
2008-03-01
影响因子：
3.5
通讯作者：
Gillenwater, Ann M.
中科院分区：
文献类型：
--
作者：
Roblyer, Darren;Richards-Kortum, Rebecca;Gillenwater, Ann M.
设计并构建了一种多光谱数码显微镜（MDM），以提高口腔肿瘤的检测水平。MDM通过荧光、窄带（NB）反射率和正交偏振反射率（OPR）模式获取口腔组织的活体图像，以便对在标准白光照射下可能不具有高对比度的病变进行评估。该设备快速捕获图像序列，以便可以单独或组合地定性和定量地评估每种模式的诊断价值。作为试点临床试验的一部分，研究人员从正常志愿者和患有癌前病变和癌前病变的患者身上获取图像。在正常受试者中，可以通过调整反射照明波长和偏振来增强血管系统的可见性。在组织学证实的肿瘤患者中，我们观察到病变内蓝/绿色自身荧光减少，红色自身荧光增加，NB和OPR成像血管可见性增加。感知到的病变边界随着成像模式的变化而变化，这表明多模式成像有可能提供标准白光照明或单独使用单一成像模式无法提供的额外诊断信息。(C) 2008光学仪器工程师学会。
A multispectral digital microscope (MDM) is designed and constructed as a tool to improve detection of oral neoplasia. The MDM acquires in vivo images of oral tissue in fluorescence, narrow-band (NB) reflectance, and orthogonal polarized reflectance (OPR) modes, to enable evaluation of lesions that may not exhibit high contrast under standard white light illumination. The device rapidly captures image sequences so that the diagnostic value of each modality can be qualitatively and quantitatively evaluated alone and in combination. As part of a pilot clinical trial, images are acquired from normal volunteers and patients with precancerous and cancerous lesions. In normal subjects, the visibility of vasculature can be enhanced by tuning the reflectance illumination wavelength and polarization. In patients with histologically confirmed neoplasia, we observe decreased blue/green autofluorescence and increased red autofluorescence in lesions, and increased visibility of vasculature using NB and OPR imaging. The perceived lesion borders change with imaging modality, suggesting that multimodal imaging has the potential to provide additional diagnostic information not available using standard white light illumination or by using a single imaging mode alone. (C) 2008 Society of Photo-Optical Instrumentation Engineers.