Cardiovascular indexes in the mouse at rest and with exercise: New tools to study models of cardiac disease
Cardiovascular indexes in the mouse at rest and with exercise: New tools to study models of cardiac disease
复制标题
DOI：
10.1152/ajpheart.1997.272.2.h1053
复制
发表时间：
1997-02-01
影响因子：
4.8
通讯作者：
Bernstein, D
中科院分区：
文献类型：
--
作者：
Desai, KH;Sato, R;Bernstein, D
对改变心血管功能的小鼠基因组的操作产生了对体内基因改造动物心血管生理学研究方法的需求。我们将慢性生理测量技术应用于非麻醉、非限制性小鼠模型，建立了品系特异性心血管和代谢规范，并评估了对麻醉、运动和肾上腺素刺激的反应。麻醉导致术后长达 6 小时的心率 (HR)、血压 (BP)、O-2 消耗 (VO2) 和 CO2 产生 (VCO2) 发生变化。静息心率和血压值存在显着的株间差异。分级跑步机运动导致 HR、VO2、VCO2 和呼吸交换比 (RER) 线性增加，与大型物种相似。对β-肾上腺素能刺激的反应显示出经典的S形剂量反应曲线；然而，对迷走神经阻滞几乎没有心动过速反应，表明静息迷走神经张力较低。这项研究证明了在非麻醉小鼠中进行慢性心血管测量的可行性，并强调了允许麻醉恢复和应变变异的重要性。小鼠心血管对运动的反应可以可靠地测量，并且在质量上与人类相似。
Manipulations of the murine genome that alter cardiovascular function have created the need for methods to study cardiovascular physiology in genetically altered animals in vivo. We adapted chronic physiological measurement techniques to the nonanesthetized, nonrestrained murine model, established strain-specific cardiovascular and metabolic norms, and evaluated responses to anesthesia, exercise, and adrenergic stimulation. Anesthesia resulted in alterations in heart rate (HR), blood pressure (BP), and O-2 consumption (VO2) and CO2 production (VCO2) for up to 6 h postoperatively. There were significant interstrain differences in resting values of HR and BP. Graded treadmill exercise resulted in linear increases in HR, VO2, VCO2, and respiratory exchange ratio (RER) similar to those seen in larger species. Response to beta-adrenergic stimulation showed a classic sigmoidal dose-response curve; however, there was very little tachycardiac response to vagal blockade, indicating low resting vagal tone. This study demonstrates the feasibility of performing chronic cardiovascular measurements in nonanesthetized mice and stresses the importance of allowing for anesthetic recovery and strain variability. Murine cardiovascular responses to exercise can be reliably measured and are qualitatively similar to those in humans.