Transcriptomic analysis of the prothoracic gland from two lepidopteran insects, domesticated silkmoth Bombyx mori and wild silkmoth Antheraea pernyi
Transcriptomic analysis of the prothoracic gland from two lepidopteran insects, domesticated silkmoth Bombyx mori and wild silkmoth Antheraea pernyi
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两种鳞翅目昆虫（家蚕家蚕和野生蚕蛾柞蚕）前胸腺的转录组分析
DOI：
10.1038/s41598-019-41864-0
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发表时间：
2019-03-29
影响因子：
4.6
通讯作者：
Qin, Li
中科院分区：
文献类型：
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作者：
Bian, Hai-Xu;Chen, Dong-Bin;Qin, Li
前胸腺是昆虫重要的内分泌器官，合成分泌蜕皮激素，在昆虫体内起着重要作用。在这里，我们使用了比较转录组学的方法来描述一些共同的特点，从两个鳞翅目物种家蚕和柞蚕的PG。功能和途径注释揭示了两个PG转录组之间的基因谱的总体相似性。正如预期的那样，几乎所有的类固醇激素生物合成基因和prothoracitropic激素受体基因（躯干）在两个PG中得到很好的代表。另外，在这两个PG中还发现了2个蜕皮激素受体基因、11个保幼激素相关基因、10多个化学感受蛋白基因和一组与生物钟有关的基因。真实的时间定量PCR（qRT-PCR）验证了8个保幼激素和12个时钟相关基因在B. moriPG基因在整个5龄幼虫发育过程中的表达模式不同。对昆虫PG转录组数据的贡献将扩展我们对这一重要器官的功能和调控的理解。
The prothoracic gland (PG) is an important endocrine organ of synthesis and secretion of ecdysteroids that play critical roles in insects. Here, we used a comparative transcriptomic approach to characterize some common features of PGs from two lepidopteran speciesBombyx moriandAntheraea pernyi. Functional and pathway annotations revealed an overall similarity in gene profile between the two PG transcriptomes. As expected, almost all steroid hormone biosynthesis genes and the prothoracicitropic hormone receptor gene (Torso) were well represented in the two PGs. Impressively, two ecdysone receptor genes, eleven juvenile hormone related genes, more than 10 chemosensory protein genes, and a set of genes involved in circadian clock were also presented in the two PGs. Quantitative real time -PCR (qRT-PCR) validated the expression of 8 juvenile hormone and 12 clock related genes inB. moriPG, and revealed a different expression pattern during development in whole fifth larval instar. This contribution to insect PG transcriptome data will extend our understanding of the function and regulation of this important organ.