The capsid protein encoded by U(L)17 of herpes simplex virus 1 interacts with tegument protein VP13/14.
The capsid protein encoded by U(L)17 of herpes simplex virus 1 interacts with tegument protein VP13/14.
复制标题
单纯疱疹病毒 1 型 U(L)17 编码的衣壳蛋白与被膜蛋白 VP13/14 相互作用。
DOI：
10.1128/jvi.00277-10
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发表时间：
2010
影响因子：
5.4
通讯作者：
Baines,JoelD
中科院分区：
文献类型：
--
作者：
Scholtes,LuellaD;Yang,Kui;Li,LucyX;Baines,JoelD
单纯疱疹病毒1型（HSV-1）的UL17蛋白（pUL17）可能与pUL25异源二聚体中含有dna的衣壳表面相关。pUL17也与缺乏衣壳蛋白的病毒轻颗粒有关，表明它存在于HSV-1病毒粒子的被膜中。为了帮助确定pUL17如何并入病毒粒子及其功能，我们在感染后16小时用pUL17特异性IgY免疫沉淀法鉴定了pUL17相互作用蛋白，然后用质谱法鉴定。共免疫沉淀蛋白包括细胞组蛋白H2A、H3和H4；中间丝蛋白波形蛋白；HSV-1主要衣壳蛋白VP5；HSV被膜蛋白VP11/12 （pUL46）和VP13/14 （pUL47）。通过在完整衣壳存在和缺失的情况下进行共免疫沉淀，以及用编码his标记的pUL17的重组病毒感染的细胞裂解物对pUL17和VP13/14进行亲和共固化，证实了pUL17-VP13/14的相互作用。在感染后9至18小时，pUL17和VP13/14-HA在细胞核复制室、细胞质和质膜上共定位。对这些数据的一种可能解释是，pUL17将衣壳的外部表面与VP13/14和相关的被皮组分连接起来。
The UL17 protein (pUL17) of herpes simplex virus 1 (HSV-1) likely associates with the surfaces of DNA-containing capsids in a heterodimer with pUL25. pUL17 is also associated with viral light particles that lack capsid proteins, suggesting its presence in the tegument of the HSV-1 virion. To help determine how pUL17 becomes incorporated into virions and its functions therein, we identified pUL17-interacting proteins by immunoprecipitation with pUL17-specific IgY at 16 h postinfection, followed by mass spectrometry. Coimmunoprecipitated proteins included cellular histone proteins H2A, H3, and H4; the intermediate filament protein vimentin; the major HSV-1 capsid protein VP5; and the HSV tegument proteins VP11/12 (pUL46) and VP13/14 (pUL47). The pUL17-VP13/14 interaction was confirmed by coimmunoprecipitation in the presence and absence of intact capsids and by affinity copurification of pUL17 and VP13/14 from lysates of cells infected with a recombinant virus encoding His-tagged pUL17. pUL17 and VP13/14-HA colocalized in the nuclear replication compartment, in the cytoplasm, and at the plasma membrane between 9 and 18 h postinfection. One possible explanation of these data is that pUL17 links the external face of the capsid to VP13/14 and associated tegument components.