bFGF as an autocrine growth factor for human melanomas.
bFGF as an autocrine growth factor for human melanomas.
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发表时间：
1988-09
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通讯作者：
R. Halaban;Kwon Bs;Sikha Ghosh;P. DelliBovi;A. Baird
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作者：
R. Halaban;Kwon Bs;Sikha Ghosh;P. DelliBovi;A. Baird
培养的正常人类黑素细胞需要特定的添加剂，如碱性成纤维细胞生长因子（bFGF）和二丁基环腺苷单磷酸（dbcAMP），以便在确定的或含血清的培养基中增殖（Halaban等，1987）。这种严格的要求在来自转移性黑色素瘤的细胞中不存在，这些细胞不仅在常规培养基中增殖，而且还产生一种与bFGF相关的免疫物质（Halaban et al, 1987）。我们在这里表明，正常人类黑素细胞必需的有丝分裂活性在几种人类转移性黑素瘤中组成性地存在，并且这种活性被抗bfgf抗体灭活。黑色素瘤细胞，而不是正常的黑色素细胞，表达bFGF基因转录物。虽然bFGF在黑色素瘤中异常表达的分子机制尚不清楚，但结果表明bFGF在黑色素瘤中起自分泌生长因子的作用。
Normal human melanocytes in culture require specific additives such as basic fibroblast growth factor (bFGF) and dibutyryl cyclic adenosine monophosphate (dbcAMP) in order to proliferate in defined or serum-containing medium (Halaban et al., 1987). This stringent requirement is absent in cells derived from metastatic melanomas which not only proliferate in regular culture medium, but also produce a substance immunologically related to bFGF (Halaban et al., 1987). We show here that the mitogenic activity necessary for normal human melanocytes is constitutively present in several lines of human metastatic melanomas and that this activity is inactivated by anti-bFGF antibodies. Melanoma cells, but not normal melanocytes, express bFGF gene transcripts. Although the molecular mechanism underlying the abnormal expression of bFGF in melanomas is not known, the results suggest that bFGF acts as an autocrine growth factor in melanomas.