TRAIL causes cleavage of bid by caspase-8 and loss of mitochondrial membrane potential resulting in apoptosis in BJAB cells.
TRAIL causes cleavage of bid by caspase-8 and loss of mitochondrial membrane potential resulting in apoptosis in BJAB cells.
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TRAIL 会导致 caspase-8 裂解 bid，并导致线粒体膜电位丧失，从而导致 BJAB 细胞凋亡。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
S. Kawanishi
中科院分区：
文献类型：
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作者：
Hiroyuki Yamada;S. Tada‐Oikawa;Atsumasa Uchida;S. Kawanishi
肿瘤坏死因子相关凋亡诱导配体（tumor necrosis factor-related apoptosis-inducing ligand，TRAIL）是肿瘤坏死因子家族的一个新成员，可诱导细胞凋亡。然而，TRAIL诱导细胞凋亡的机制仍有待阐明。SDS-PAGE和Western blot分析表明，BJAB细胞与TRAIL孵育1小时诱导Bid裂解，并被caspase-8抑制剂阻断。流式细胞术显示，BJAB细胞中的线粒体膜电位的损失开始后约1.5小时与TRAIL的处理，并在2小时与对照相比是明显的。在用TRAIL处理的细胞中，在2 h检测到DNA梯状条带形成，这是凋亡的特征，并且在3 h观察到最有效。时程研究表明，TRAIL通过激活caspase-8引起Bid裂解，随后线粒体膜电位丧失，导致BJAB细胞凋亡。
A new member of the TNF family, tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), has been shown to induce apoptosis. However, the mechanism for TRAIL-induced apoptosis remains to be clarified. SDS-PAGE and Western blot analysis showed that cleavage of Bid was induced by a 1-h incubation of BJAB cells with TRAIL and was blocked by a caspase-8 inhibitor. Flow cytometry demonstrated that loss of mitochondrial membrane potential in BJAB cells began about 1.5 h after the treatment with TRAIL and was apparent at 2 h in comparison with the control. DNA ladder formation, which is characteristic for apoptosis, in the cells treated with TRAIL was detected at 2 h and observed most effectively at 3 h. The time course study suggests that TRAIL causes cleavage of Bid via activation of caspase-8, subsequently the loss of mitochondrial membrane potential, resulting in apoptosis in BJAB cells.
影响因子：
10.5
作者：
Wang, K;Yin, XM;Korsmeyer, SJ
通讯作者：
Korsmeyer, SJ