A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats.
A palatable hyperlipidic diet causes obesity and affects brain glucose metabolism in rats.
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DOI：
10.1186/1476-511x-10-168
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发表时间：
2011-09-23
影响因子：
4.5
通讯作者：
Oller do Nascimento CM
中科院分区：
文献类型：
--
作者：
Estadella D;Oyama LM;Bueno AA;Habitante CA;Souza GI;Ribeiro EB;Motoyama CS;Oller do Nascimento CM
我们以前已经表明，无论是持续摄入可口的高脂饮食（H）或食物（C）和H饮食（CH方案）的交替诱导大鼠肥胖。在这里，我们调查了这些喂养方案的开始时间和持续时间是否相关，以及它们是否影响大脑葡萄糖代谢。雄性Wistar大鼠在其生命周期的不同时期接受C、H或CH饮食：第30-60天、第30-90天或第60-90天。实验在出生后第60天或第90天进行。通过断头处死大鼠。测定了尸体的葡萄糖、胰岛素、瘦素血浆浓度和脂质含量。将脑切片并与或不与胰岛素一起孵育，以分析葡萄糖摄取、氧化和[1- 14 C]-葡萄糖向脂质的转化。高脂组和高脂组的相对脂肪含量均有所增加，其中以H30-60和H30-90组的相对脂肪含量最高。组H30-60、H30-90、CH 30 -60和CH 30 -90显示较高的血清葡萄糖水平。血清瘦素在所有H组和CH 60 -90和CH 30 -90组均升高。H30-60、H60-90、CH 60 - 90、CH 30 -90组血清胰岛素水平升高。基础脑葡萄糖消耗和下丘脑胰岛素受体密度仅在CH 30 -60组中较低。H30-90和CH 30 -90组的脑脂肪生成率增加。这些发现表明，H和CH饮食方案增加了身体肥胖独立治疗和开始治疗的年龄，而这些饮食在早期开始时会引起高血糖并影响大脑代谢。
We have previously shown that either the continuous intake of a palatable hyperlipidic diet (H) or the alternation of chow (C) and an H diet (CH regimen) induced obesity in rats. Here, we investigated whether the time of the start and duration of these feeding regimens are relevant and whether they affect brain glucose metabolism. Male Wistar rats received C, H, or CH diets during various periods of their life spans: days 30-60, days 30-90, or days 60-90. Experiments were performed the 60th or the 90th day of life. Rats were killed by decapitation. The glucose, insulin, leptin plasma concentration, and lipid content of the carcasses were determined. The brain was sliced and incubated with or without insulin for the analysis of glucose uptake, oxidation, and the conversion of [1-14C]-glucose to lipids. The relative carcass lipid content increased in all of the H and CH groups, and the H30-60 and H30-90 groups had the highest levels. Groups H30-60, H30-90, CH30-60, and CH30-90 exhibited a higher serum glucose level. Serum leptin increased in all H groups and in the CH60-90 and CH30-90 groups. Serum insulin was elevated in the H30-60, H60-90, CH60-90, CH30-90 groups. Basal brain glucose consumption and hypothalamic insulin receptor density were lower only in the CH30-60 group. The rate of brain lipogenesis was increased in the H30-90 and CH30-90 groups. These findings indicate that both H and CH diet regimens increased body adiposity independent treatment and the age at which treatment was started, whereas these diets caused hyperglycemia and affected brain metabolism when started at an early age.