The expression of free oligosaccharides in human seminal plasma
The expression of free oligosaccharides in human seminal plasma
复制标题
DOI：
10.1074/jbc.m205152200
复制
发表时间：
2002-09-06
影响因子：
4.8
通讯作者：
Clark, GF
中科院分区：
文献类型：
--
作者：
Chalabi, S;Easton, RL;Clark, GF
人类精浆是由男性生殖道器官分泌的蛋白质、糖蛋白、多肽、糖肽和前列腺素的复杂混合物。这种液体的成分涉及抑制免疫反应，对精子-卵子结合的激动作用，以及促进人类胚胎的成功着床。生物物理分析表明，游离寡糖是精浆中总糖缀合物的主要组成部分。我们的分析有以下重要发现：(i)游离低聚糖的浓度为0.3-0.4 mg/ml；（ii）单浓缩和二浓缩形式的双糖乳糖是主要成分；（iii）许多剩余的寡糖也富含焦点并携带Lewis(x)和/或Lewis(y)表位；（iv）低聚糖的一个子集表达在人乳低聚糖中未报道的还原端序列（GlcNAcbeta1-3/4Glc）；(v)精浆中的寡糖只表达2型（Galbeta1-4GlcNAc），而不表达在人乳聚糖中占主导地位的1型序列（Ga1beta1-3GlcNAc）；精浆寡糖的结构多样性远不如人乳寡糖。在体外实验中，聚焦糖缀合物和聚焦糖缀合物对人精卵结合的激动作用表明，聚焦糖缀合物也可能促进女性生殖道的受精。
Human seminal plasma is a complex mixture of proteins, glycoproteins, peptides, glycopeptides, and prostaglandins secreted by organs of the male reproductive tract. The components of this fluid have been implicated in the suppression of immune response, agonistic effects on sperm-egg binding, and promotion of successful implantation of the human embryo. Fractionation followed by biophysical analyses revealed that free oligosaccharides constitute a major component of the total glycoconjugates within seminal plasma. Significant findings of our analyses include the following: (i) the concentration of free oligosaccharides is 0.3-0.4 mg/ml; (ii) mono and difucosylated forms of the disaccharide lactose are major components; (iii) many of the remaining oligosaccharides are also rich in fucose and carry Lewis(x) and/or Lewis(y) epitopes; (iv) a subset of the oligosaccharides express the reducing end sequence (GlcNAcbeta1-3/4Glc) not reported in human milk oligosaccharides; (v) oligosaccharides in seminal plasma exclusively express type 2 (Galbeta1-4GlcNAc) but not the type 1 sequences (Ga1beta1-3GlcNAc) that predominate in human milk glycans; and (vi) the structural diversity of seminal plasma oligosaccharides is far less than human milk oligosaccharides. The agonistic effect of both fucose and fucosylated glycoconjugates on human sperm-egg binding in vitro suggests that fucosylated oligosaccharides may also promote fertilization in the female reproductive tract.