A dual functional peptide carrying in vitro selected catalytic and binding activities
A dual functional peptide carrying in vitro selected catalytic and binding activities
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具有体外选择性催化和结合活性的双功能肽
DOI：
10.1039/c5ob01271f
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
and Yoshihiro Ito
中科院分区：
文献类型：
--
作者：
Liping Zhu;Wei Wang;Haixu Zhao;Muye Xu;Seiichi Tada;Takanori Uzawa;Mingzhe Liu;and Yoshihiro Ito
当使用最小的功能序列时，可以在单个肽链上整合多种功能，就像“单笔画”一样。在此，通过结合体外选择的催化和结合活性来设计双功能肽。对于催化活性，我们通过使用核糖体展示对结合氯化血红素的肽适体进行体外选择，并分离出在氯化血红素存在下具有过氧化物酶活性的肽。通过将所选择的催化肽与可被抗体识别的肽抗原结合，获得酶-抗体缀合物样肽。本研究证明了一种成功的策略，以创建用于免疫测定的双官能化肽链。
When minimal functional sequences are used, it is possible to integrate multiple functions on a single peptide chain, like a “single stroke drawing”. Here a dual functional peptide was designed by combining in vitro selected catalytic and binding activities. For catalytic activity, we performed in vitro selection for a peptide aptamer binding to hemin by using ribosome display and isolated a peptide that had peroxidase activity in the presence of hemin. By combining the selected catalytic peptide with a peptide antigen, which can be recognized by an antibody, an enzyme–antibody conjugate-like peptide was obtained. This study demonstrates a successful strategy to create dual functionalized peptide chains for use in immunoassays.