Effect of Electroporation on Cell Killing by Boron Neutron Capture Therapy Using Borocaptate Sodium (10B‐BSH)
Effect of Electroporation on Cell Killing by Boron Neutron Capture Therapy Using Borocaptate Sodium (10B‐BSH)
复制标题
电穿孔对硼中子捕获疗法（10B-BSH）细胞杀伤的影响
DOI：
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
M. Takagaki
中科院分区：
文献类型：
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作者：
K. Ono;Y. Kinashi;S. Masunaga;Minoru Suzuki;M. Takagaki
富含 10B 的硼己酸钠 (BSH) 的细胞膜渗透性以及 BSH 在细胞中积累的程度存在争议。为了阐明这些观点并增强 BSH 在细胞中的积累，研究了电穿孔对使用 BSH 的硼中子捕获疗法 (BNCT) 的影响。第一组 SCCVII 肿瘤细胞在含有 10B-BSH 或富含 10B 的硼酸的培养基中孵育，并暴露于来自京都大学反应堆重水设施的中子。在通量基础上，99%以上的中子是热中子。第二组采用电穿孔结合10B-BSH进行预处理，然后用中子照射细胞。 The cell‐killing effect of BNCT was measured by colony formation assay.存活细胞分数随中子注量呈指数下降，并且添加 BSH 显着增强了 NCT 的细胞杀伤效果，具体取决于 10B 浓度和细胞在含有 BSH 的培养基中的预孵育时间。与单独预孵育相比，用 BSH 电穿孔细胞显着增强了 BNCT 效果。电穿孔的 BSH-BNCT 效果几乎与相同 10B 浓度下使用 10B-硼酸的 BNCT 效果相同。 BNCT 对用 BSH 和电穿孔预处理的细胞的影响不会因中子照射前重复洗涤细胞而减弱。随着电穿孔和中子照射之间等待时间的增加，BSH-BNCT 加电穿孔的效果下降，这可以用这段时间内细胞生长的程度来解释。这些数据表明 BSH 缓慢渗透细胞并在洗涤后保留下来。电穿孔可以非常有效地将BSH引入细胞中，并且由此引入的BSH保留在细胞中，并且尽管强烈洗涤细胞也不会丢失。因此，如果在注射BSH后对肿瘤进行电穿孔，10B将保留在肿瘤中，而从正常组织中被清除，并在适当的等待时间后实现10B在肿瘤中的选择性积累。
The cell membrane permeability of 10B‐enriched borocaptate sodium (BSH) and the extent to which BSH is accumulated in cells are controversial. To elucidate these points and to enhance the accumulation of BSH in cells, the effect of electroporation on boron neutron capture therapy (BNCT) using BSH was investigated. The first group of SCCVII tumor cells was incubated in culture medium with 10B‐BSH or 10B‐enriched boric acid, and exposed to neutrons from the heavy water facility of the Kyoto University Reactor. More than 99% of neutrons were thermal neutrons at flux base. The second group was pretreated with electroporation in combination with 10B‐BSH, and thereafter the cells were irradiated with neutrons. The cell‐killing effect of BNCT was measured by colony formation assay. The surviving cell fraction decreased exponentially with neutron fluence, and addition of BSH significantly enhanced the cell‐killing effect of NCT depending on 10B concentration and the preincubation time of cells in the BSH‐containing culture medium. The electroporation of cells with BSH markedly enhanced the BNCT effect in comparison with that obtained with preincubation alone. The effect of BSH‐BNCT with electroporation was almost equal to that of BNCT using 10B‐boric acid at the same 10B concentration. The effect of BNCT on cells pretreated with BSH and electroporation was not reduced by repeated washing of the cells before neutron irradiation. Decrease of the effect of BSH‐BNCT plus electroporation with increase in the waiting time between the electroporation and the neutron irradiation could be explained in terms of the extent of cell growth during that time. These data suggest that BSH penetrates the cells slowly and remains after washing. Electroporation can introduce BSH into the cells very efficiently, and BSH thus introduced stays in the cells and is not lost in spite of the intensive washing of the cells. Therefore, if electroporation is applied to tumors after BSH injection, 10B would remain in the tumors but be cleared from normal tissues, and selective accumulation of 10B in tumors will be achieved after an appropriate waiting time.
用于中子捕获治疗的硼化化合物的吸收、保留、分布、生物功效和毒性的体外测定：卟啉与巯基硼氢化物的比较。
影响因子：
11.2
作者：
Fairchild,RG;Kahl,SB;Laster,BH;Kalef-Ezra,J;Popenoe,EA
通讯作者：
Popenoe,EA
体内消耗谷胱甘肽作为提高米索硝唑和 SR 2508 治疗比例的方法。
DOI：
10.1016/0360-3016(84)90330-4
发表时间：
1984
期刊：
International journal of radiation oncology, biology, physics
影响因子：
--
作者：
Yu,NY;Brown,JM
通讯作者：
Brown,JM