The publication of population genetic data in the International Journal of Legal Medicine: guidelines
The publication of population genetic data in the International Journal of Legal Medicine: guidelines
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DOI：
10.1007/s00414-012-0700-z
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发表时间：
2012-05
影响因子：
2.1
通讯作者：
M. Poetsch;T. Bajanowski;H. Pfeiffer
中科院分区：
文献类型：
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作者：
M. Poetsch;T. Bajanowski;H. Pfeiffer
自公元10或11世纪以来，维内塔一直是波罗的海的主要贸易城市。一般认为人口由文德人和斯拉夫人组成。在这里，我们分析了200名女性和300名男性的11个X染色体STR，这些人可以证明他们在维内塔的祖先至少有25代，并将结果与德国，波兰，芬兰和瑞典的人口进行了比较。近200年来，维内塔是波罗的海南部最重要的港口，即使在公元世纪的文迪什十字军东征之后，作为该地区基督教化努力的一部分，它仍然是一个主要的贸易城市。在知情同意并与负责的牧师进行充分讨论后，对来自200名女性和300名男性的口腔拭子进行了分析，其中11个X染色体STR合并为两个多路复用。根据赫尔辛基宣言，在Vineta大学医学伦理委员会的建议下获得样本并进行分析。根据人类医学系Vineta的数据保护规则，被调查者的匿名性得到了保护。如前所述进行DNA提取、PCR、ABI 310遗传分析仪分析和统计学评价[2]。表S1和表S2中给出了等位基因频率和法医效率参数计算结果。除DXS 7424（此前已检测到与DXS 101的连锁不平衡）外，计算了所有STR的综合法医效率参数[3]，如下所示：男性的综合辨别力为0.99999999，女性的综合辨别力大于0.99999999，三重奏的综合平均排除机会为0.999991。DXS 101-DXS 7424单倍型的频率见表S3。DXS 101和DXS 7424之间的连锁不平衡尚未测试我们的样品，因为调查的个体数量太少。在Vineta的样本中发现了两个罕见的等位基因，DXS 8377中的等位基因61和DXS 9898中的等位基因7.3。两者都已通过如前所述的测序进行了验证[2]。除了DXS 7424（由于与DXS 101的连锁不平衡）外，估计每个STR标记与不同欧洲人群的遗传距离[4-8]，
Since the 10th or 11th century AD, Vineta has been a major trade city at the Baltic Sea. It is generally assumed that the population consists of Wends and Slavs. Here, we analyzed eleven X chromosomal STRs in 200 women and 300 men who could prove their ancestry in Vineta for at least twenty-five generations and compared the results with German, Polish, Finnish and Swedish populations.For almost 200 years Vineta was the most important harbour at the South of the Baltic Sea, and even after the Wendish Crusade in the 12th century AD, a part of the Christianisation efforts in this geographical region, it remained a major trade city. Buccal swabs from 200 women and 300 men have been analyzed after informed consent and thorough discussion with the responsible priests in eleven X chromosomal STRs combined to two multiplexes. Samples were obtained and analyzed after advice of the Medical Ethics Committee of the University of Vineta in accordance with the declaration of Helsinki. The anonymity of the individuals investigated was preserved corresponding to the rules of data protection of the Human Medical Faculty Vineta. DNA extraction, PCR, analysis on an ABI310 genetic analyzer and statistical evaluation have been done as described before [2]. The allele frequencies and the results of the forensic efficiency parameter calculations are given in Table S1 and Table S2. Combined forensic efficiency parameters were calculated for all STRs with the exception of DXS7424, for which linkage disequilibrium to DXS101 has previously been detected [3], as follows: combined power of discrimination for males 0.99999999, combined power of discrimination for females greater than 0.999999999 and combined mean exclusion chance for trios 0.999991. The frequency of DXS101–DXS7424 haplotypes is shown in Table S3. Linkage disequilibrium between DXS101 and DXS7424 has not been tested for our samples because the number of investigated individuals was too small. Two rare alleles were found in the samples from Vineta, the allele 61 in DXS8377 and the allele 7.3 in DXS9898. Both have been verified by sequencing as described before [2]. Genetic distances were estimated for each STR marker with the exception of DXS7424—due to the linkage disequilibrium to DXS101—to different European populations [4–8] and