SYNTHETIC PEPTIDES BASED ON MOTIFS PRESENT IN HUMAN BAND-3 PROTEIN INHIBIT CYTOADHERENCE SEQUESTRATION OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM
SYNTHETIC PEPTIDES BASED ON MOTIFS PRESENT IN HUMAN BAND-3 PROTEIN INHIBIT CYTOADHERENCE SEQUESTRATION OF THE MALARIA PARASITE PLASMODIUM-FALCIPARUM
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DOI：
10.1073/pnas.90.10.4703
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发表时间：
1993-05-15
影响因子：
11.1
通讯作者：
SHERMAN, IW
中科院分区：
文献类型：
--
作者：
CRANDALL, I;COLLINS, WE;SHERMAN, IW
在条带3蛋白的两个外表面区域（环7的824-829残基和环3的547-553残基）中发现的氨基酸序列上的合成肽以剂量依赖的方式阻断了恶性疟原虫感染的红细胞对C32无色素黑色素瘤细胞的体外粘附。将这些合成肽静脉输注到感染了恶性疟原虫分离株的猕猴和猴体内，导致外周循环中出现成熟形式的疟原虫。这些肽在微摩尔范围内作为黏附阻滞剂有效的发现表明，脑型疟疾可以通过抗黏附治疗来控制。
Synthetic peptides patterned on the amino acid sequences found in two exofacial regions of band 3 protein (residues 824-829 of loop 7 and residues 547-553 of loop 3) blocked, in a dose-dependent fashion, the in vitro adherence of Plasmodium falciparum-infected erythrocytes to C32 amelanotic melanoma cells. Intravenous infusion of these synthetic peptides into Aotus and Saimiri monkeys infected with sequestering isolates of P. falciparum resulted in the appearance of mature forms of the parasite in the peripheral circulation. The finding that the peptides were effective as adhesion blockers in the micromolar range suggests that cerebral malaria could be managed through antiadhesion therapy.