Investigation of Tissue-Specific Human Orthologous Alternative Splice Events in Pig
Investigation of Tissue-Specific Human Orthologous Alternative Splice Events in Pig
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DOI：
10.1080/10495398.2010.497729
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发表时间：
2010-10
影响因子：
3.7
通讯作者：
A. Nygård;C. Jørgensen;S. Cirera;M. Fredholm
中科院分区：
文献类型：
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作者：
A. Nygård;C. Jørgensen;S. Cirera;M. Fredholm
前体mRNA的选择性剪接可以通过调节基因表达或产生具有由一个基因编码的各种功能的蛋白质来促成组织或细胞之间的差异。到目前为止，猪中研究的选择性剪接事件的数量有限。在这项研究中，我们已经调查了在人类中检测到的选择性剪接事件，在orthopathic猪基因。共选择了17个具有预测外显子跳跃事件的基因进行进一步研究。在19种不同组织中使用剪接特异性引物的实时定量PCR分析（qPCR）对所选基因的剪接事件进行了实验验证。在15个正位猪基因中检测到与人类中报告的相同的剪接变体，然而，在计算机模拟分析中预测的表达模式仅在少数情况下得到实验验证。结果支持了以下发现：导致开放阅读框保留的剪接事件指示基因剪接变体的功能意义。
Alternative splicing of pre-mRNA can contribute to differences between tissues or cells either by regulating gene expression or creating proteins with various functions encoded by one gene. The number of investigated alternative splice events in pig has so far been limited. In this study we have investigated alternative splice events detected in humans, in orthologous pig genes. A total of 17 genes with predicted exon skipping events were selected for further studies. The splice events for the selected genes were experimentally verified using real-time quantitative PCR analysis (qPCR) with splice-specific primers in 19 different tissues. The same splice variants as reported in humans were detected in 15 orthologous pig genes, however, the expression pattern predicted in the in silico analyses was only experimentally verified in a few cases. The results support the findings that splice events resulting in preservation of open reading frame are indicative of a functional significance of the splice variants of the gene.