Analysis of pressure/flow characteristics in the female rat and their pharmacologic modulation
Analysis of pressure/flow characteristics in the female rat and their pharmacologic modulation
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DOI：
10.1002/(sici)1520-6777(1996)15:5
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发表时间：
1996
影响因子：
2
通讯作者：
Takeshi Watanabe;C. Constantinou
中科院分区：
文献类型：
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作者：
Takeshi Watanabe;C. Constantinou
开发了一种新的体内尿动力学动物模型来分析大鼠的排尿特征。该模型用于利用膀胱测压法和尿流率测定法来研究药物对膀胱尿道功能的调节作用。对 25 只用乌拉坦麻醉的雌性大鼠进行了压力流量研究。使用通过经膀胱输注的膀胱充盈的生理速率来记录充盈性膀胱测压。通过确定排尿时间和排尿量来表征排尿。通过机械传感器的新颖应用来测量排泄体积，该应用提供了测量流速和计算排泄体积-时间曲线的数据。通过对机械传感器产生的曲线进行微分来计算流量。使用该系统，使用抗胆碱能刺激、α1 阻滞剂和新型 N-甲基-D-天冬氨酸 (NMDA) 受体拮抗剂进行药理刺激的比较测试。在不同剂量水平下评估了这些药物在下尿路静脉内使用的效果。结果表明，抗胆碱能刺激导致膀胱容量增加，逼尿肌压力和最大流量降低。尽管α1阻滞剂降低了逼尿肌压力，但流速没有显着变化。相比之下，NMDA 受体拮抗作用对膀胱反射性收缩产生抑制作用，并以剂量依赖性方式增加膀胱容量。然而，最大流速在剂量为 10 mg/kg 时显着增加，而在剂量为 30 mg/kg 时显着下降。这些结果表明，通过出口区域的流阻降低是由于较低剂量下 NMDA 受体抑制的影响。总之，该模型能够评估有关下尿路功能的药物，并为小动物提供评估各种实验模型中压力和流量之间关系的可能性。 © 1996 Wiley-Liss, Inc.
A new in vivo urodynamic animal model was developed to analyze the micturition characteristics of the rat. This model was used to study the modulating effect of pharmacological agents on vesicourethral function, using cystometry and uroflowmetry. Pressure‐flow studies were done in 25 female rats anesthetized with urethane. Filling cystometry was recorded using a physiological rate of bladder filling through transvesical infusion. Micturition characterization was done by identifying the time course and amount of voided volume. Voided volume was measured by a novel application of a mechanotransducer, which provided the data to measure flow rate and compute the voided volume‐time curve. Flow rate was calculated by differentiating the curve produced by the mechanotransducer. Using this system, comparative tests of pharmacological stimulus were done using anticholinergic stimulation, α1 blocker, and a new N‐methyl‐D‐aspartate (NMDA) receptor antagonist. The effects of the intravenous use of these drugs in the lower urinary tract were evaluated at various dose levels. The results showed that anticholinergic stimulation produced an increase of bladder capacity and decreases of detrusor pressure and maximum flow rate. Although the α1 blocker decreased detrusor pressure, flow rate did not change significantly. By contrast, NMDA receptor antagonism produced a depressant effect on bladder reflex contraction, and increased bladder capacity in a dose‐dependent way. However, maximum flow rate increased at a dose of 10 mg/kg and decreased at 30 mg/kg significantly. These results suggest that a decrease in flow resistance through the outlet region was due to the effects of NMDA receptor inhibition at lower doses. In conclusion, this model enables the evaluation of drugs regarding lower urinary tract function and provides in small animals the possibility of evaluating the relationships between pressure and flow in various experimental models. © 1996 Wiley‐Liss, Inc.