Expressing the modification of diet in renal disease study equation for estimating glomerular filtration rate with standardized serum creatinine values
Expressing the modification of diet in renal disease study equation for estimating glomerular filtration rate with standardized serum creatinine values
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DOI：
10.1373/clinchem.2006.077180
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发表时间：
2007-04-01
影响因子：
9.3
通讯作者：
Van Lente, Frederick
中科院分区：
文献类型：
--
作者：
Levey, Andrew S.;Coresh, Josef;Van Lente, Frederick
目的：我们试图用标准化的参考方法重新表达用于估计肾小球滤过率的四变量修正膳食研究方程(S-cr)。方法：血清标本包括由美国病理学家学会(CAP)准备的可追溯到NIST的主要参考物质的肌酐参考物质，其指定值可追溯到同位素稀释质谱仪(IDMS)、克利夫兰临床研究实验室(CCRL)准备的校准小组以及来自MDRD研究的冰冻样本。裂解标本在CCRL用罗氏酶和Beckman CX3动态碱性苦味酸法测定。结果：罗氏酶对CAP样品的测定结果与IDMS指定值相当。2004-2005年Beckman CX3检测结果显著高于同期检测结果，但与同期结果高度相关。罗氏酶分析结果(40个CCRL样本的r(2)=0.9994)与1989年至1991年MDRD研究期间Beckman CX3的结果有微小但显著的上升(r(2)=0.9987)。综合这些因素，标准化S-cr=0.95X原始MDRD研究S-cr。重新表达的S-cr(mg/dL)的四变量MDRD研究方程为：GfR=30849 X标准化的S-cr(-1.154)X年龄(-0.203)x 1.212(如果是黑人)×0.742(如果是女性)；对于S-cr(单位：L)，GfR=175X标准化的S-cr(-1.154)X年龄(-0.203)X 1.212(如果是黑人)X 0.742(如果是女性)[GfR(单位：毫升)。分钟(-1)。(1.73m(2)(-1))]结论：当S-cr方法的校正可追溯到S-cr参考系时，应使用重新表示为标准S-cr的MDRD研究方程来估计肾小球滤过率。(C)2007年美国临床化学协会。
Purpose: We sought to reexpress the 4-variable Modification of Diet in Renal Disease (MDRD) Study equation for estimation of glomerular filtration rate (GFR) using serum creatinine (S-cr) standardized to reference methods.Methods: Serum specimens included creatinine reference materials prepared by the College of American Pathologists (CAP), traceable to primary reference material at the NIST, with assigned values traceable to isotope dilution mass spectrometry (IDMS), a calibration panel prepared by the Cleveland Clinic Research Laboratory (CCRL), and frozen samples from the MDRD Study. Split specimens were measured at the CCRL using the Roche enzymatic and Beckman CX3 kinetic alkaline picrate assays.Results: Roche enzymatic assay results on CAP samples were comparable to IDMS-assigned values. Beckman CX3 assay results in 2004-2005 were significantly higher than but highly correlated with simultaneous. Roche enzymatic assay results (r(2) = 0.9994 on 40 CCRL samples) and showed minimal but significant upward drift from Beckman CX3 assay results during the MDRD Study in 1989-1991 (r(2) = 0.9987 in 253 samples). Combining these factors, standardized S-cr = 0.95 X original MDRD Study S-cr. The reexpressed 4-variable MDRD Study equation for S-cr (mg/dL) is GFR = 175 X standardized S-cr(-1.154) X age(-0.203) x 1.212 (if black) X 0.742 (if female), and for S-cr (mu mol/L) is GFR = 30849 X standardized S-cr(-1.154) X age(-0.203) X 1.212 (if black) X 0.742 (if female) [GFR in mL . min(-1) . (1.73 m(2))(-1)].Conclusion: When the calibration Of S-cr methods is traceable to the S-cr reference system, GFR should be estimated using the MDRD Study equation that has been reexpressed for standardized S-cr. (c) 2007 American Association for Clinical Chemistry.