Assessing reproductive status/stages in mice.
Assessing reproductive status/stages in mice.
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评估小鼠的生殖状态/阶段。
DOI：
10.1002/0471142301.nsa04is48
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发表时间：
2009-07
影响因子：
--
通讯作者：
Caligioni, Claudia S
中科院分区：
文献类型：
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作者：
Caligioni, Claudia S
在啮齿动物身上观察到的较短的生殖周期长度称为发情周期，这使它们成为研究生殖周期变化的理想动物模型。文献中关于发情周期的大部分数据都是从大鼠身上获得的，因为它们很容易操作，并且表现出明确和明确的发情周期。然而，使用基因敲除小鼠进行的实验数量增加也需要确定它们的发情周期，因为可能会出现生育问题。在小鼠中，像大鼠一样，动情周期阶段的识别是基于在阴道分泌物中观察到的细胞类型的比例。本单元的目的是为快速准确地确定小鼠的发情周期阶段提供指南。
The short reproductive cycle length observed in rodents, called the estrous cycle, makes them an ideal animal model for investigation of changes that occur during the reproductive cycle. Most of the data in the literature about the estrous cycle is obtained from rat because they are easily manipulated and exhibit a clear and well defined estrous cycle. However, the increased number of experiments using knockout mice requires identification of their estrous cycle as well, since (in)fertility issues may arise. In mice, like rats, the identification of the stage of estrous cycle is based on the proportion of cells types observed in the vaginal secretion. The aim of this unit is to provide guidelines for quickly and accurately determining estrous cycle phases in mice.