The Molecular Events Involved in Oligodendrocyte Precursor Cell Proliferation Induced by the Conditioned Medium from B104 Neuroblastoma Cells
The Molecular Events Involved in Oligodendrocyte Precursor Cell Proliferation Induced by the Conditioned Medium from B104 Neuroblastoma Cells
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B104神经母细胞瘤细胞条件培养基诱导少突胶质前体细胞增殖的分子事件
DOI：
10.1007/s11064-012-0957-0
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发表时间：
2013-03-01
影响因子：
4.4
通讯作者：
Lu, He-Zuo
中科院分区：
文献类型：
--
作者：
Hu, Jian-Guo;Wu, Xing-Jun;Lu, He-Zuo
B104神经母细胞瘤细胞（B104 CM）的条件培养基在体外诱导少突胶质祖细胞（OPCs）增殖。然而，在B104 CM诱导的OPCs增殖过程中发生的分子事件尚未得到很好的阐明。在本研究中，使用从胚胎第14天的Sprague-Dawley大鼠脊髓免疫接种的OPCs，我们探讨了B104 CM对OPCs中几种信号通路的激活和几种重要的立即早期基因（IEGs）和细胞周期蛋白的表达的影响。我们发现B104 CM可以通过激活细胞外信号调节激酶1和2（Erk 1/2）而不是PI 3 K或p38 MAPK信号通路诱导OPC增殖。参与B104 CM诱导的OPC增殖的IEG包括c-fos、c-jun和Id 2，但不包括c-myc、fyn或p21。细胞周期蛋白D1、D2和E也参与B104 CM刺激的OPCs增殖。Erk的激活导致随后的IEGs（如c-fos、c-jun和Id-2）和cyclin（包括cyclin D1、D2和E）的表达，其在细胞周期启动和OPC增殖中起关键作用。综上所述，这些结果表明，Erk 1/2的磷酸化是B104 CM诱导的OPC增殖过程中的重要分子事件。
The conditioned medium from B104 neuroblastoma cells (B104CM) induces proliferation of oligodendrocyte progenitor cells (OPCs) in vitro. However, the molecular events that occur during B104CM-induced proliferation of OPCs has not been well clarified. In the present study, using OPCs immunopanned from embryonic day 14 Sprague–Dawley rat spinal cords, we explored the activation of several signaling pathways and the expression of several important immediate early genes (IEGs) and cyclins in OPCs in response to B104CM. We found that B104CM can induce OPC proliferation through the activation of the extracellular signal-regulated kinases 1 and 2 (Erk1/2), but not PI3K or p38 MAPK signaling pathways in vitro. The IEGs involved in B104CM-induced OPC proliferation include c-fos, c-jun and Id2, but not c-myc, fyn, or p21. The cyclins D1, D2 and E are also involved in B104CM-stimulated proliferation of OPCs. The activation of Erk results in subsequent expression of IEGs (such as c-fos, c-jun and Id-2) and cyclins (including cyclin D1, D2 and E), which play key roles in cell cycle initiation and OPC proliferation. Collectively, these results suggest that the phosphorylation of Erk1/2 is an important molecular event during OPC proliferation induced by B104CM.