Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method
Simultaneous determination of eight catechins and four theaflavins in green, black and oolong tea using new HPLC-MS-MS method
复制标题
DOI：
10.1016/j.jpba.2016.08.020
复制
发表时间：
2016-11-30
影响因子：
3.4
通讯作者：
Wei, Tongyu
中科院分区：
文献类型：
--
作者：
Tao, Wuqun;Zhou, Zhiguang;Wei, Tongyu
建立了一种简单、快速、准确的分析方法，采用超高效液相色谱-三重四极杆串联质谱（HPLC-MS-MS）在多反应监测（MRM）模式下同时分析了3种茶叶（绿色、红茶和乌龙茶）中的8种茶儿茶素和4种茶黄素。在CAPCELL PAK C18 MGIII上以正离子模式使用HPLC-MS-MS进行该方法（2.0 mm x 100 mm，3 μ m）色谱柱（Shiseido），移动的相由0.1%甲酸水溶液（A）和甲醇（B）组成，以0.3 mL min（-1）的流速梯度洗脱，优化的HPLC-MS-MS方法具有选择性和特异性，并对不同茶中广泛报道的八种儿茶素和四种茶黄素进行了验证。儿茶素和茶黄素的线性范围分别为0.02-5 μ gmL（-1）和0.02-20 μ gmL（-1），相关系数r（2）> 0.9935。回收率为65%~ 115%，相对标准偏差为2.4%~ 6.7%。采用该方法对三种茶叶样品中的目标多酚含量进行了评价。(C)2016爱思唯尔B. V.保留所有权利。
A simple, rapid and accurate analytical method was developed for the analysis of eight tea catechins and four theaflavins simultaneously in three types of tea (green, black and oolong tea), using ultra high performance liquid chromatography coupled with triple-quadrupole tandem mass spectrometry (HPLC-MS-MS) in multiple-reaction monitoring (MRM) mode. This method using HPLC-MS-MS in positive mode was performed on a CAPCELL PAK C18 MGIII (2.0 mm x 100 mm, 3 mu m) column (Shiseido) with the mobile phase consisting of 0.1% aqueous formic acid (A) and methanol (B) in gradient elution ion at a flow rate of 03 mL min(-1), and the column temperature set at 30 degrees C The optimized HPLC-MS-MS methodology is selective and specific, and was validated for eight catechins and four theaflavins widely reported in different teas. Satisfactory linearity was achieved in linear range (0.02-5 mu gmL(-1) for catechins and 0.02-20 mu gmL(-1) for theaflavins) and fine determination coefficient (r(2) > 0.9935). The recoveries ranged from 65% to 115% with the RSD ranging from 2.4% to 6.7%. The methodology was used to evaluate the target polyphenols concentration in three types of tea samples. (C) 2016 Elsevier B.V. All rights reserved.