Leukemic Stem Cell Culture in Cytokine-Free Medium.
Leukemic Stem Cell Culture in Cytokine-Free Medium.
复制标题
在无细胞因子培养基中培养白血病干细胞。
DOI：
10.1007/978-1-0716-0810-4_15
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Klein,PeterS
中科院分区：
文献类型：
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作者：
Liu,Xiaolei;Klein,PeterS
白血病起始细胞，也称为白血病干细胞（LSC），是通过其植入免疫功能低下小鼠的能力而被实验定义的，并且被认为是急性髓性白血病（AML）复发的主要原因。尽管急性白血病具有侵袭性特征，但 AML 母细胞一旦从患者体内取出就很难培养，而 LSC 只占母细胞群体的一小部分，培养后尤其难以移植。这阻碍了针对 LSC 的 AML 新疗法的开发。在这里，我们提出了一种简单的策略，在无细胞因子的培养基中培养 LSC，并对所得细胞群进行流式细胞术分析，以表征 LSC 的维持和分化。
Leukemia-initiating cells, also known as leukemic stem cells (LSCs), are experimentally defined by their ability to engraft immunocompromised mice and are believed to be a major cause of relapse in acute myeloid leukemia (AML). Despite the aggressive characteristics of acute leukemia, AML blasts are difficult to culture once removed from the patient, and LSCs, which are a minor fraction of the blast population, are especially difficult to transplant after culture. This impedes development of new therapies for AML that target LSCs. Here, we present a simple strategy to culture LSCs in cytokine-free medium and to perform flow cytometric analysis of the resulting cell population for the characterization of LSCs maintenance and differentiation.