A model of transcriptional and morphological changes during thyroid hormone-induced metamorphosis of the axolotl.
A model of transcriptional and morphological changes during thyroid hormone-induced metamorphosis of the axolotl.
复制标题
DOI：
10.1016/j.ygcen.2009.03.001
复制
发表时间：
2009-06
影响因子：
2.7
通讯作者：
Voss, S. Randal
中科院分区：
文献类型：
--
作者：
Page, Robert B.;Monaghan, James R.;Walker, John A.;Voss, S. Randal
关键词：
无尾蛙(蛙)的变态长期以来一直被认为是甲状腺激素如何调节脊椎动物胚胎后发育的模型。然而，人们对蜥蜴的变态知之甚少。我们对墨西哥南方红豆杉(Ambystoma Micianum)的诱导变态进行了详细的时间进程研究，探讨了皮肤形态和基因表达的变态变化。利用形态计量学、定量聚合酶链式反应、组织学和原位杂交技术，我们证明转录标记的发展是解决胚轴早期变质事件的基础。然后，我们使用线性和分段线性模型来识别一系列的形态和转录变化，这些变化定义了整个变态过程中幼虫到成虫的重塑事件。此外，我们发现转录生物标记物在特定的幼虫和成体皮肤细胞群中表达，这些生物标记物的时间变化与组织重塑相关。我们将我们的结果与其他关于尾龙自然和诱导变态的研究进行了比较，并强调了尾龙和无尾两栖动物变态之间似乎具有保守的特征。
Anuran (frog) metamorphosis has long-served as a model of how thyroid hormones regulate post-embryonic development in vertebrates. However, comparatively little is known about urodele (salamander) metamorphosis. We conducted a detailed time-course study of induced metamorphosis in the Mexican axolotl (Ambystoma mexicanum) that probed metamorphic changes in morphology and gene expression in the skin. Using morphometrics, quantitative PCR, histology, and in situ hybridization we demonstrate that the development of transcriptional markers is fundamental to the resolution of early metamorphic events in axolotls. We then use linear and piecewise linear models to identify a sequence of morphological and transcriptional changes that define larval to adult remodeling events throughout metamorphosis. In addition, we show that transcriptional biomarkers are expressed in specific larval and adult cell populations of the skin and that temporal changes in these biomarkers correlate with tissue remodeling. We compare our results with other studies of natural and induced metamorphosis in urodeles and highlight what appear to be conserved features between urodele and anuran metamorphosis.
登录
查看更多内容
影响因子：
3.5
作者：
BenPorath, I;Benvenisty, N
通讯作者：
Benvenisty, N
影响因子：
2.7
作者：
GALTON, VA
通讯作者：
GALTON, VA
影响因子：
2.7
作者：
DARRAS, VM;KUHN, ER
通讯作者：
KUHN, ER
影响因子：
2.7
作者：
DUCIBELLA, T
通讯作者：
DUCIBELLA, T
影响因子：
2.7
作者：
Furlow, JD;Berry, DL;Brown, DD
通讯作者：
Brown, DD