Age-dependent consequences of seizures and the development of temporal lobe epilepsy in the rat
Age-dependent consequences of seizures and the development of temporal lobe epilepsy in the rat
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DOI：
10.1159/000046147
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发表时间：
2001-05-01
影响因子：
2.9
通讯作者：
Nehlig, A
中科院分区：
文献类型：
--
作者：
Dubé, C;Fernandes, MJDS;Nehlig, A
癫痫发生中与年龄相关的功能改变仍有待阐明。在本研究中，我们探讨了锂-匹罗卡品（Li-Pilo）在10-龄（P10）、21日龄（P21）和成年大鼠癫痫持续状态（SE）后急性、沉默和慢性期代谢变化、神经元损伤与癫痫发生的相关性。采用[C-14]2-脱氧葡萄糖法测定SE、沉默期和慢性期间期的局部脑葡萄糖代谢率（LCMRglcs）。甲酚紫染色评估神经变性。SE期间，LCMRglcs在各年龄段均显著升高，主要发生在前脑易损区。在沉默期，在P21和成年大鼠中，在SE后14天，在与癫痫发作发生和传播有关的受损前脑区域记录到代谢减少。在沉默期结束时，P21和成年大鼠在参与癫痫远程控制的完整脑干区域表现出代谢增加。在慢性期的间歇期，成年大鼠和非自发癫痫的P21大鼠的受损前脑区域LCMRglcs下降，而癫痫P21大鼠的LCMRglcs与对照水平相似。在P10大鼠中，SE后的任何时间都没有损伤和代谢后果。综上所述，P21大鼠与成年大鼠的癫痫发生过程及其功能后果存在差异。这些与年龄相关的差异背后的因素仍有待探索。版权所有(C) 2001 S. Karger AG，巴塞尔
The age-related functional changes underlying epileptogenesis remain to be clarified. In the present study, we explored the correlation between metabolic changes, neuronal damage and epileptogenesis during the acute, silent and chronic phases following status epilepticus (SE) induced by lithium-pilocarpine (Li-Pilo) in 10- (P10), 21-day-old (P21) and adult rats. Local cerebral metabolic rates for glucose (LCMRglcs) were measured by the [C-14]2-deoxyglucose method during SE, the silent period and the interictal phase of the chronic period. Neurodegeneration was assessed by cresyl violet staining. During SE, LCMRglcs dramatically increased at all ages mainly in forebrain vulnerable regions. During the silent phase, in P21 and adult rats, metabolic decreases were recorded in damaged forebrain regions involved in the genesis and propagation of seizures 14 days after SE. At the end of the silent phase, P21 and adult rats exhibited metabolic increases in intact brainstem areas involved in the remote control of epilepsy. During the interictal phase of the chronic period, LCMRglcs decreased in damaged forebrain areas of adult and P21 rats that were not spontaneously epileptic, while LCMRglcs were similar to control levels in epileptic P21 rats. In P10 rats, there was no damage and no metabolic consequences at any time after SE. In conclusion, the process of epileptogenesis and its functional consequences differ in P21 and adult rats. The factors underlying these age-related differences remain to be explored. Copyright (C) 2001 S. Karger AG, Basel.