2 TYPES OF NEURONS IN MYENTERIC PLEXUS OF DUODENUM IN GUINEA-PIG
2 TYPES OF NEURONS IN MYENTERIC PLEXUS OF DUODENUM IN GUINEA-PIG
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DOI：
10.1113/jphysiol.1974.sp010436
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发表时间：
1974-01-01
影响因子：
5.5
通讯作者：
SPENCE, I
中科院分区：
文献类型：
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作者：
HIRST, GDS;HOLMAN, ME;SPENCE, I
1.细胞内记录已经从位于豚鼠十二指肠肌间神经丛的神经元中进行;通过使电流通过细胞内电极同时记录膜电位的变化来研究其膜特性的某些方面。在膜电位变化较小的情况下，电流-电压关系呈线性，输入电阻范围为125 - 250 MΩ。较大的超极化电流（引起20-40 mV的变化）引起输入电阻下降。1至10 × 10−10A的去极化电流引发幅度高达95 mV的动作电位。两种类型的细胞区分时，动作电位启动。在一组中，动作电位和下冲的形式与其他哺乳动物神经节记录的相似。在第二组中，动作电位之后是下冲和延长的后超极化，这与细胞电阻的降低有关.通过对透壁刺激的反应进一步区分这两组细胞。只有那些没有表现出后超极化的细胞才能被证明接受突触输入。每种细胞类型产生动作电位的机制是不同的。从细胞记录的动作电位有一个可检测的突触输入被河豚毒素废除。与此相反，从其他类型的细胞记录持续存在的河豚毒素。初步实验表明，在动作电位期间，这些细胞变得对钠离子和钙离子都具有渗透性。7.消除产生后超极化的细胞中动作电位的钙成分消除了后一种电位。这两组细胞的作用进行了讨论。
1. Intracellular recordings have been made from neurones lying in the myenteric plexus of guinea‐pig duodenum; some aspects of their membrane properties have been studied by passing current through the intracellular electrode while recording changes in membrane potential.2. The current—voltage relationship was linear for small changes in membrane potential, input resistances ranging from 125 to 250 MΩ. Larger hyperpolarizing currents (causing changes of 20–40 mV) caused the input resistance to fall.3. Depolarizing currents of 1 to 10 × 10−10A initiated action potentials with amplitudes of up to 95 mV.4. Two types of cell were distinguished when an action potential was initiated. In one group the action potential and undershoot had a form similar to that recorded from other mammalian ganglia. In the second group an action potential was followed by both an undershoot and a prolonged afterhyperpolarization which was associated with a decrease in cell resistance.5. The two groups of cells were further distinguished by their responses to transmural stimulation. Only those cells which did not show an afterhyperpolarization could be shown to receive a synaptic input.6. The mechanism by which each cell type generates an action potential was different. The action potentials recorded from cells which had a detectable synaptic input were abolished by tetrodotoxin. In contrast, those recorded from the other type of cells persisted in the presence of tetrodotoxin. Preliminary experiments suggest that during an action potential these cells become permeable to both sodium and calcium ions.7. Abolition of the calcium component of the action potential in cells which generated an afterhyperpolarization abolished this latter potential.8. The role of these two groups of cells is discussed.