Behavioral functions and hippocampal cell types: evidence for two nonoverlapping populations in the rat.
Behavioral functions and hippocampal cell types: evidence for two nonoverlapping populations in the rat.
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DOI：
10.1152/jn.1986.55.2.331
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发表时间：
1986-02
影响因子：
2.5
通讯作者：
E. Christian;S. Deadwyler
中科院分区：
文献类型：
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作者：
E. Christian;S. Deadwyler
细胞外记录的行为大鼠海马神经元进行了分类的几个不同的电生理标准为θ（θ-）或复杂的锋细胞。在两种不同的行为背景下研究了每个细胞的放电特征，其中每种行为背景下都包含先前已经证明这些细胞的放电相关性的实验条件。对48个θ细胞和68个复合棘细胞进行了分类和电生理研究，动物进行了操作性听觉辨别任务或自由探索开放平台环境的水奖励。自发放电的特点，以及电诱发的放电模式刺激海马纤维通路，一贯分化θ-细胞从复杂的棘细胞。与以前的报告一致，这些结果表明，θ细胞和复杂的棘细胞代表电生理和可能形态不同类型的海马神经元。一个明确的二分法之间也出现了确定θ细胞和复杂的棘细胞在两个行为测试的发射相关。在辨别学习和操作过程中，条件性行为反应的出现与θ-细胞对声调起始的时间锁定放电有关。在空间探索任务的运动过程中也观察到θ细胞的活性增加。锁定时间的增加，在射击的音调刺激被限制到确定θ细胞。复杂的棘细胞，在相反的θ-细胞，并没有增加活动在任何阶段的音调辨别学习或性能。然而，在探索平台期间，相同的细胞以显著增加的速率与动物在特定空间位置的存在相一致，但在平台上的其他明显行为（如梳理，饮水或运动）期间没有发射。根据阳性碰撞试验和逆行标准，复合棘突细胞符合海马输出神经元的条件。“空间放电”是复杂的棘突细胞所特有的，在同一动物的θ细胞中没有观察到。在这两种行为背景下研究的21个θ细胞和31个复杂的棘细胞表现出相互排斥和非重叠的电生理放电相关性。在这两个类别中没有观察到细胞表现出空间和音调辨别相关的放电相关性。（400字处截断摘要）
Extracellular recordings of individual hippocampal neurons in behaving rats were classified by several different electrophysiological criteria as theta (theta-) or complex spike cells. The firing characteristics of each cell were studied in two different behavioral contexts, each of which contained experimental conditions in which firing correlates of these cells have been demonstrated previously. Forty-eight theta-cells and 68 complex spike cells were classified and studied electrophysiologically as animals performed an operant auditory discrimination task or freely explored an open platform environment for water reward. Spontaneous discharge characteristics, as well as electrically evoked firing patterns following stimulation of hippocampal fiber pathways, consistently differentiated theta-cells from complex spike cells. In agreement with previous reports, these results indicate that theta-cells and complex spike cells represent electrophysiologically and possibly morphologically different types of hippocampal neurons. A clear dichotomy also emerged between the firing correlates of identified theta-cells and complex spike cells in the two behavioral tests. During auditory-discrimination learning and performance, time-locked theta-cell discharges to tone onset were correlated with the emergence of conditioned behavioral responding. Increased activity of theta-cells was also observed during locomotion in the spatial exploration task. Time-locked increases in firing to the tone stimulus were restricted to identified theta-cells. Complex spike cells, in contrast to theta-cells, did not increase activity during any phase of tone-discrimination learning or performance. The same cells, however, fired at markedly increased rates coincident with the animals' presence in specific spatial locations during exploration of the platform but did not fire during other overt behaviors on the platform such as grooming, drinking, or locomotion. Complex spike cells qualified on the basis of positive collision tests and antidromic criteria as hippocampal output neurons. "Spatial firing" was unique to complex spike cells and was not observed from theta-cells recorded from the same animal. Twenty-one theta-cells and 31 complex spike cells studied in both behavioral contexts showed mutually exclusive and nonoverlapping electrophysiological firing correlates. No cell was observed in either category which exhibited spatial and tone discrimination related firing correlates.(ABSTRACT TRUNCATED AT 400 WORDS)