Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil
Modifications of PARP medium using fluazinam, miconazole, and nystatin for detection of Pythium spp. in soil
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DOI：
10.1094/pdis-91-12-1591
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发表时间：
2007-12-01
期刊：
影响因子：
4.5
通讯作者：
Tojo, M.
中科院分区：
文献类型：
--
作者：
Morita, Y.;Tojo, M.
对腐霉标准选择性培养基PARP（匹马菌素+氨苄青霉素+利福平+五氯硝基苯[PCNB]琼脂）进行了改良，用其它抗真菌剂代替PCNB和匹马菌素。几种抗真菌剂，如氟啶胺，咪康唑，2，4，5，6-四氯氰（TPN），三乙酸亚胺霉胺，甲基立枯磷，克菌丹，制霉菌素，最初筛选的腐霉生长的影响。根据这些结果，开发了以下三种培养基：PRF（匹马菌素+氨苄青霉素+利福平+氟啶胺琼脂）、NARF（制霉菌素+氨苄青霉素+利福平+氟啶胺琼脂）和NARM（制霉菌素+氨苄青霉素+利福平+咪康唑琼脂）。新培养基与PARP在自然发生腐霉属的产量上相当。从两种不同类型的土壤使用土壤稀释平板技术。PRF和NARF对土壤稀释平板上的非腐霉微生物的抑制作用显著优于PARP，但对7种腐霉属8个菌株中的6个菌株的菌丝生长速率显著低于PARP。NARM对除尖孢镰刀菌外的其他非腐霉菌的抑制作用与PARP相当，对8个腐霉菌菌株中的5个菌株的菌丝生长速率显著优于PARP。
The standard Pythium selective medium PARP (pimaricin + ampicillin + rifampicin + pentachloronitrobenzene [PCNB] agar), was modified by replacing PCNB and pimaricin with other antifungal agents. Several antifungal agents such as fluazinam, miconazole, 2,4,5,6-tetrachloroisophthalonitrile (TPN), iminoctadine triacetate, tolclofos-methyl, captan, and nystatin, initially were screened for effects on Pythium growth. Based on these results, the following three media were developed: PARF (pimaricin + ampicillin + rifampicin + fluazinam agar), NARF (nystatin + ampicillin + rifampicin + fluazinam agar), and NARM (nystatin + ampicillin + rifampicin + miconazole agar). New media were comparable with PARP on yield of naturally occurring Pythium spp. from two different types of soil using the soil-dilution plating technique. PARF and NARF were significantly better than PARP on inhibition of non-pythiaceous microbes on the soil-dilution plates, but were significantly lower than PARP on the rate of mycelial growth of six of eight isolates belonging to seven species of Pythium. NARM was equivalent to PARP on inhibition of non-pythiaceous microbes except for Fusarium oxysporum, and was significantly better than PARP on rate of mycelial growth of five of eight isolates of Pythium.