Macromolecular Crowding Effects on Reactions of TePixD (Tll0078) †
Macromolecular Crowding Effects on Reactions of TePixD (Tll0078) †
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大分子拥挤对 TePixD (Tll0078) 反应的影响 †
DOI：
10.1111/j.1751-1097.2010.00849.x
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发表时间：
2011
影响因子：
3.3
通讯作者：
M. Terazima
中科院分区：
文献类型：
--
作者：
Tsuguyoshi Toyooka;Keisuke Tanaka;K. Okajima;M. Ikeuchi;S. Tokutomi;M. Terazima
为了揭示来自嗜热蓝藻热共生球菌（Thermosynechococcus elongatus） BP‐1的BLUF（利用FAD的蓝光传感器）蛋白（PixD）化学反应中的大分子拥挤效应[TePixD， tl0078])，利用紫外/可见吸收光谱和脉冲激光诱导瞬态光栅（TG）方法研究了不同浓度的大分子Ficoll‐70的光反应。吸收光谱不随Ficoll‐70浓度的变化而变化。拥挤不影响光谱红移反应的量子产率、产物的回收率、体积变化反应的速率常数和体积变化的大小。然而，在加入Ficoll‐70后，代表扩散敏感构象变化的TG信号的幅度显著增加。这种依赖性归因于溶液中TePixD十聚体-五聚体平衡的拥挤效应。这一结果表明TePixD在细胞条件下的反应比在体外条件下更有效。
To reveal macromolecular crowding effects on a chemical reaction of a BLUF (sensors of blue light using FAD) protein (PixD from a thermophilic cyanobacterium Thermosynechococcus elongatus BP‐1 [TePixD, Tll0078]), the photoreaction was studied at various concentrations of the macromolecule Ficoll‐70 by UV/Vis absorption spectroscopy and the pulsed laser‐induced transient grating (TG) method. The absorption spectrum did not change with varying concentration of Ficoll‐70. The crowding did not affect the quantum yield of the spectral red shift reaction, recovery rate of the product, rate constant of the volume change reaction and the magnitude of the volume change. However, the magnitude of the TG signal representing the diffusion‐sensitive conformation change significantly increased on addition of Ficoll‐70. This dependence was attributed to the crowding effect on the TePixD decamer–pentamer equilibrium in the solution. This result indicates that the TePixD reaction is more efficient in cellular than in in vitro conditions.
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影响因子：
3.4
作者：
Qin, Sanbo;Zhou, Huan-Xiang
通讯作者：
Zhou, Huan-Xiang
影响因子：
5.6
作者：
Sasahara, K;McPhie, P;Minton, AP
通讯作者：
Minton, AP
影响因子：
2.9
作者：
Yuan, Hua;Anderson, Spencer;Bauer, Carl
通讯作者：
Bauer, Carl
影响因子：
3.4
作者：
Rösgen, J;Pettitt, BM;Bolen, DW
通讯作者：
Bolen, DW