NITRILE HYDRATASE-CATALYZED PRODUCTION OF NICOTINAMIDE FROM 3-CYANOPYRIDINE IN RHODOCOCCUS-RHODOCHROUS J1
NITRILE HYDRATASE-CATALYZED PRODUCTION OF NICOTINAMIDE FROM 3-CYANOPYRIDINE IN RHODOCOCCUS-RHODOCHROUS J1
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DOI：
10.1128/aem.54.7.1766-1769.1988
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发表时间：
1988-07-01
影响因子：
4.4
通讯作者：
YAMADA, H
中科院分区：
文献类型：
--
作者：
NAGASAWA, T;MATHEW, CD;YAMADA, H
腈水合酶大量存在于从土壤样品中分离出的红球菌 J1 细胞中，可催化 3-氰基吡啶水合为烟酰胺。通过使用静息细胞，优化了烟酰胺生产的反应条件。在最佳条件下，添加的12 M 3-氰基吡啶100%转化为烟酰胺，且不形成烟酸，最高产量为每升含有静息细胞的反应混合物（细胞干重1.48 g）在9 h内获得1,465 g烟酰胺。将产生的烟酰胺结晶，然后进行物理化学鉴定。烟酰胺进一步转化为烟酸是由于烟酰胺作为该生物体中存在的酰胺酶的底物的活性较低。
Nitrile hydratase, which occurs abundantly in cells of Rhodococcus rhodochrous J1 isolated from soil samples, catalyzes the hydration of 3-cyanopyridine to nicotinamide. By using resting cells, the reaction conditions for nicotinamide production were optimized. Under the optimum conditions, 100% of the added 12 M 3-cyanopyridine was converted to nicotinamide without the formation of nicotinic acid, and the highest yield achieved was 1,465 g of nicotinamide per liter of reaction mixture containing resting cells (1.48 g as dry cell weight) in 9 h. The nicotinamide produced was crystallized and then identified physicochemically. The further conversion of the nicotinamide to nicotinic acid was due to the low activity of nicotinamide as a substrate for the amidase(s) present in this organism.