Retinal glial cell responses and Fas/FasL activation in rats with chronic ocular hypertension
Retinal glial cell responses and Fas/FasL activation in rats with chronic ocular hypertension
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DOI：
10.1016/j.brainres.2006.09.022
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发表时间：
2006-11-29
期刊：
影响因子：
2.9
通讯作者：
Park, Chan Kee
中科院分区：
文献类型：
--
作者：
Ju, Kwany Ro;Kim, Hwa Sun;Park, Chan Kee
损伤后视网膜的反应会引发神经胶质反应，但目前尚不完全清楚。本研究调查了慢性高眼压大鼠视网膜神经胶质细胞的反应以及 Fas 和 Fas 配体 (FasL) 的表达和定位。通过烧灼三个巩膜外层血管，在 60 只 Sprague-Dawley 大鼠的一只眼睛中诱发实验性青光眼。它导致所有动物中度眼压 (IOP) 升高和显着视网膜神经节细胞 (RGC) 损失至少 6 周。免疫组织化学分析显示，受损视网膜中 GFAP 和 OX-42 的表达增加。小胶质细胞中的 Fas/FasL 免疫反应性升高，我们还观察到 IOP 升高的视网膜中的米勒胶质细胞和 RGC 中 Fas 相关死亡结构域 (FADD) 免疫反应性逐渐增加。胶质细胞的激活以及Fas和FasL的上调表明，在慢性高眼压视网膜损伤的神经变性过程中，胶质细胞可能导致Fas介导的细胞死亡。 (c) 2006 Elsevier B.V. 保留所有权利。
Responses in the retina post injury provoke glial reactions that are not completely understood. This study investigated the reaction of retinal glial cells and the expression and localization of the Fas and Fas-ligand (FasL) in rats with chronic ocular hypertension. Experimental glaucoma was induced in one eye of 60 Sprague-Dawley rats by cauterizing three episcleral vessels. It caused a moderate intraocular pressure (IOP) elevation and significant retinal ganglion cell (RGC) loss for at least 6 weeks in all animals. Immunohistochemical analysis revealed that the expression of GFAP and OX-42 increased in the injured retinae. Fas/FasL immunoreactivity was elevated in the microglia, and we also observed an incremental increase in Fas associated death domain (FADD) immunore activity in Milller glial cells and RGCs in the IOP-elevated retinae. The activation of glial cells and upregulation of Fas and FasL suggest that glial cells may contribute to Fas-mediated cell death in the neuro degeneration process of chronic ocular hypertensive retinal insult. (c) 2006 Elsevier B.V. All rights reserved.