Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment
Comparison of real-time polymerase chain reaction and DNA-strip technology in microbiological evaluation of periodontitis treatment
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DOI：
10.1016/j.diagmicrobio.2010.08.017
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发表时间：
2011-01-01
影响因子：
2.9
通讯作者：
Jentsch, Holger
中科院分区：
文献类型：
--
作者：
Eick, Sigrun;Straube, Anna;Jentsch, Holger
与定量内部实时PCR相比，评估了基于DNA条技术的半定量市售测试（microlDent（R），海恩生命科学公司，Nehren，德国）对25名牙周炎患者的严重慢性牙周炎的诊断和治疗的影响。在基线以及3个月、6个月和12个月后收集龈下菌斑样本。提取DNA后，Aggregatibacteractinomycetemcomitans，牙龈卟啉单胞菌，Tannerellaeasthia，密螺旋体denticola，和其他几种牙周病病原体确定由两种方法。通过DNA条带技术获得的结果进行了半定量分析，并通过光密度法进行了额外的定量分析。两种方法对4种主要牙周致病菌的检测结果具有显著相关性。通过一种方法检测到高细菌负荷而通过另一种方法检测到阴性的样品总是在总样品的不到2%中发现。两种技术都显示了处理对微生物区系的影响。特别是半定量DNA条带技术清楚地分析了治疗后牙周致病菌的不同载量，并可用于牙科实践中患者的微生物诊断。(C)2011 Elsevier Inc. All rights reserved.
The impact of a semiquantitative commercially available test based on DNA-strip technology (microlDent (R), Hain Lifescience, Nehren, Germany) on diagnosis and treatment of severe chronic periodontitis of 25 periodontitis patients was evaluated in comparison with a quantitative in-house real-time PCR. Subgingival plaque samples were collected at baseline as well as at 3, 6, and 12 months later. After extracting DNA, Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and several other periodontopathogens were determined by both methods. The results obtained by DNA-strip technology were analyzed semiquantitatively and additionally quantitatively by densitometry. The results for the 4 major periodontopathogenic bacterial species correlated significantly between the 2 methods. Samples detecting a high bacterial load by one method and negative by the other were always found in less than 2% of the total samples. Both technologies showed the impact of treatment on microflora. Especially the semiquantitative DNA-strip technology clearly analyzed the different loads of periodontopathogens after therapy and is useful in microbial diagnostics for patients in dental practices. (C) 2011 Elsevier Inc. All rights reserved.