The murine B cell repertoire responsive to an influenza-infected syngeneic cell line.
The murine B cell repertoire responsive to an influenza-infected syngeneic cell line.
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DOI：
10.4049/jimmunol.127.1.194
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发表时间：
1981-07
影响因子：
4.4
通讯作者：
D. Wylie;N. Klinman
中科院分区：
文献类型：
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作者：
D. Wylie;N. Klinman
定义了诱导单克隆B细胞对流感感染的同源肿瘤细胞系应答的条件。虽然感染的肿瘤细胞刺激强烈的反应，很少有克隆识别肿瘤抗原，而超过90%的单克隆抗体识别病毒抗原表达在感染的肿瘤细胞表面。大约三分之一的病毒特异性抗体与病毒血凝素（HA）或神经氨酸酶反应，其余的识别感染细胞表面上的抗原，但不识别病毒粒子或未感染细胞上的抗原。大多数抗体与病毒体反应的HA决定簇，而很少与神经氨酸酶反应。随后的分析表明，大多数神经氨酸酶决定簇暴露在完整的病毒体上，而在感染细胞的表面上是不可用的。相比之下，大多数的HA决定簇上的病毒体上表达的也可在感染细胞的表面膜上，和一个高度多样化的抗体组识别的HA上表达的病毒体或感染的细胞表面上。最后，在响应于感染的细胞与纯化的病毒产生的抗体的重链同种型中注意到差异。大多数纯化的流感病毒的单克隆反应包括伊加抗体，而感染细胞的反应显示较少的伊加和伴随的增加表达IgG的克隆。
The conditions for eliciting monoclonal B cell responses to an influenza-infected syngeneic tumor cell line were defined. Although the infected tumor cell stimulated vigorous responses, few clones recognized tumor antigens, whereas over 90% of the monoclonal antibodies recognized viral antigens expressed on the surface of infected tumor cells. Approximately one-third of the virus-specific antibodies reacted with the viral hemagglutinin (HA) or neuraminidase, and the remainder recognized antigens found on the surface of infected cells but not on the virion or uninfected cells. The majority of the antibodies reactive with the virion recognized HA determinants, whereas few reacted with the neuraminidase. Subsequent analysis revealed that most neuraminidase determinants exposed on the intact virion were not available on the surface of infected cells. In contrast, the majority of the HA determinants expressed on the virion were also available on the surface membranes of infected cells, and a highly diverse set of antibodies recognized the HA expressed either on the virion or on the infected cell surface. Finally, differences were noted in the heavy chain isotype of antibodies produced in response to infected cells vs purified virus. A majority of monoclonal responses to purified influenza virus included IgA antibodies, whereas responses to infected cells showed less IgA and a concomitant increase in clones expressing IgG.